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Antibodies > APOB / Apolipoprotein B > LS-C355399
 
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Catalog Number Size Price
LS-C355399-1 1 mg $857 
APOB / Apolipoprotein B Antibody - Immunohistochemistry analysis of Apolipoprotein B showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Apolipoprotein B Mouse Monoclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
APOB / Apolipoprotein B Antibody - Immunofluorescence analysis of Apolipoprotein B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein B (F2C3) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
APOB / Apolipoprotein B Antibody - Immunohistochemistry analysis of Apolipoprotein B showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Apolipoprotein B Mouse Monoclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
APOB / Apolipoprotein B Antibody - Immunofluorescence analysis of Apolipoprotein B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein B (F2C3) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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Monoclonal Mouse anti‑Human APOB / Apolipoprotein B Antibody (clone F2C3, IHC, IF) LS‑C355399

Monoclonal Mouse anti‑Human APOB / Apolipoprotein B Antibody (clone F2C3, IHC, IF) LS‑C355399

Submit Review LSBio Guarantee
Antibody:
APOB / Apolipoprotein B Mouse anti-Human Monoclonal (F2C3) Antibody
Application:
IHC, IF, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355399-1
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APOB / Apolipoprotein B Mouse anti-Human Monoclonal (F2C3) Antibody
Application:
IHC, IF, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Apolipoprotein B antibody LS-C355399 is an unconjugated mouse monoclonal antibody to human Apolipoprotein B (APOB). Validated for ELISA, IF, IHC, IP and RIA.
Target
Human APOB / Apolipoprotein B
Synonyms
APOB | ApoB-100 | Apolipoprotein B-48 | ApoB-48 | Apolipoprotein B | FLDB | LDLCQ4 | Mutant Apo B 100 | Apo B-100 | Apolipoprotein B-100 | Apolipoprotein B48
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
F2C3
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Purified human serum LDL.
Specificity
The antibody targets Apolipoprotein B in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Apolipoprotein B which has a predicted molecular weight of approximately 513 kD.
Applications
  • IHC
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOB / Apolipoprotein B
P04114 NM_000384 NP_000375.2

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Featured Products

APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ APOB / Apolipoprotein B Antibody LS‑B6180
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot, Immunodiffusion
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ APOB / Apolipoprotein B Antibody LS‑B3646
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
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APOB / Apolipoprotein B Antibody LS‑C20713
Species: Human
Applications: Western blot, Immunoprecipitation
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
IHC‑plus™ APOB / Apolipoprotein B Antibody (aa3984‑3995) LS‑B3022
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APOB / Apolipoprotein B Antibody (aa4031‑4080) LS‑B4653
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
APOB / Apolipoprotein B Antibody - Anti-APOB / Apolipoprotein B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ APOB / Apolipoprotein B Antibody (clone GF1) LS‑B11205
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
See all APOB / Apolipoprotein B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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