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APOB / Apolipoprotein B

apolipoprotein B

Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence. In the ApoB-100 isoform the precursor has 4563 amino acids, and the mature apoB-100 has 4536 amino acid residues. Mature, circulating B-48 is homologous over its entire length (estimated to be between 2130 and 2144 amino acid residues) with the amino-terminal portion of B-100 and contains no sequence from the carboxyl end of B-100. From structural studies, it is thought that apoB-48 represents the amino-terminal 47% of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of apoB-100. Apolipoprotein B-48 may be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C-to-U change in the codon CAA encoding Gln(2153) in apoB-100 mRNA. Since only the sequence that codes B-100 is present in genomic DNA, this presents the possibility of an organ-specific introduction of a stop codon to an mRNA and the change from CAA to UAA of codon 2153 of the message as a unique RNA editing process.

Gene Name: apolipoprotein B
Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48
Target Sequences: NM_000384 NP_000375.2 P04114

Publications (7)

1
Apolipoprotein B48, the Structural Component of Chylomicrons, Is Sufficient to Antagonize Staphylococcus aureus Quorum-Sensing. Elmore BO, Triplett KD, Hall PR. PloS one. 2015 10:e0125027. (WB; Mouse) [Full Text Article] [PubMed:25942561] [PMC:PMC4420250] Related Antibodies: LS-B6180.
2
Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Zhu L, Shi J, Luu TN, Neuman JC, Trefts E, Yu S, Palmisano BT, Wasserman DH, Linton MF, Stafford JM. Molecular metabolism. 2018 February;8:106-116. [Full Text Article] [PubMed:29331506] [PMC:PMC5985047] Related Antibodies: LS-B6180.
3
Spatial distributions of lipids in atherosclerosis of human coronary arteries studied by time-of-flight secondary ion mass spectrometry. Lehti S, Sjövall P, Käkelä R, Mäyränpää MI, Kovanen PT, Öörni K. The American journal of pathology. 2015 May;185:1216-1233. [Full Text Article] [PubMed:25805604]
4
Extracellular Lipids Accumulate in Human Carotid Arteries as Distinct Three-Dimensional Structures and Have Proinflammatory Properties. Satu Lehti, Su D Nguyen, Ilya Belevich, Helena Vihinen, Hanna M Heikkilä, Rabah Soliymani, Reijo Käkelä, Jani Saksi, Matti Jauhiainen, Gregory A Grabowski, Outi Kummu, Sohvi Hörkkö, Marc Baumann, Perttu J Lindsberg, Eija Jokitalo, Petri T Kovanen, Katariina Öörni. The American journal of pathology. 2018 Feb;188:525-538. [Full Text Article] [PubMed:29154769]
5
Estrogen-related receptor ? regulates hepatic triglyceride metabolism through phospholipase A2 G12B. Longhui Chen, Min Wu, Shengnan Zhang, Wenjuan Tan, Min Guan, Liqiang Feng, Chen Chen, Jun Tao, Ling Chen, Linbing Qu. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 Jul;33:7942-7952. [Full Text Article] [PubMed:30922124]
6
Estrogen signaling prevents diet-induced hepatic insulin resistance in male mice with obesity. Zhu L, Martinez MN, Emfinger CH, Palmisano BT, Stafford JM. American journal of physiology. Endocrinology and metabolism. 2014 May;306:E1188-97. [Full Text Article] [PubMed:24691030] [PMC:PMC4116406] Related Antibodies: LS-B6180.
7
Estrogen treatment after ovariectomy protects against fatty liver and may improve pathway-selective insulin resistance. Zhu L, Brown WC, Cai Q, Krust A, Chambon P, McGuinness OP, Stafford JM. Diabetes. 2013 Feb;62:424-34. [Full Text Article] [PubMed:22966069] [PMC:PMC3554377] Related Antibodies: LS-B6180.
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Antibodies (122)
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APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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APOB / Apolipoprotein B Rabbit anti-Mouse Polyclonal Antibody
Mouse, Human
ID, IHC, IHC-P, WB
Unconjugated
0.05 ml/$525
APOB / Apolipoprotein B Antibody - Anti-APOB / Apolipoprotein B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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APOB / Apolipoprotein B Mouse anti-Human Monoclonal (GF1) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
0.5 mg/$505
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
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APOB / Apolipoprotein B Goat anti-Human Polyclonal (aa3984-3995) Antibody
Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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APOB / Apolipoprotein B Goat anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated
50 µg/$685
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APOB / Apolipoprotein B Goat anti-Human Polyclonal Antibody
IP, WB
Unconjugated
1 ml/$523
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APOB / Apolipoprotein B Rabbit anti-Human Polyclonal (aa4031-4080) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APOB / Apolipoprotein B Rabbit anti-Human Polyclonal (aa4031-4080) (FITC) Antibody
Human, Gibbon
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
6.25 - 400 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$883
APOB / Apolipoprotein B ELISA Kit
Select
Sandwich
96-Well Strip Plate
Rat
6.25 - 400 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
APOB / Apolipoprotein B ELISA Kit
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Competitive EIA
96-Well Microplate
Mouse
0.156 - 10 µg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$734
APOB / Apolipoprotein B ELISA Kit
Select
Sandwich
96-Well Microplate
Human
78.13 - 5000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$528
APOB / Apolipoprotein B ELISA Kit
Select
Sandwich
96-Well Microplate
Mouse
31.25 - 2000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$495
APOB / Apolipoprotein B ELISA Kit
Select
Sandwich
96-Well Strip Plate
Mouse
6.25 - 400 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$748
APOB / Apolipoprotein B Protein - Recombinant Apolipoprotein B By SDS-PAGE
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E. coli
His
19.6 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
APOB / Apolipoprotein B Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
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APOB / Apolipoprotein B Mouse anti-Human Monoclonal Antibody
Human
IHC, IHC-P
Unconjugated
0.5 mg/$460
APOB / Apolipoprotein B Antibody - Immunofluorescence analysis of Apolipoprotein B was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein B (F2C9) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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APOB / Apolipoprotein B Mouse anti-Human Monoclonal (F2C9) Antibody
Human
ELISA, IF, IP, RIA
Unconjugated
1 mg/$913
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APOB / Apolipoprotein B Mouse anti-Human Monoclonal (B1) Antibody
Human
ELISA, RIA
Unconjugated
500 µg/$826
APOB / Apolipoprotein B Antibody - Western Blot; Sample: Recombinant protein.
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APOB / Apolipoprotein B Rabbit anti-Mouse Polyclonal (aa3360-3526) Antibody
Mouse
WB
Unconjugated
100 µl/$311; 200 µl/$355; 500 µl/$499; 1 ml/$571; 2 ml/$777; 5 ml/$1,261
APOB / Apolipoprotein B Antibody - Immunofluorescence of HepG2 cells using ApoB mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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APOB / Apolipoprotein B Mouse anti-Human Monoclonal (6G6) Antibody
Human
ELISA, Flo, IF, WB
Unconjugated
100 µl/$459
APOB / Apolipoprotein B ELISA Kit
Select
Sandwich
96-Well Microplate
Human
2.458 - 600 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum
1 Plate/$635
APOB / Apolipoprotein B Protein - Recombinant Apolipoprotein B By SDS-PAGE
Select
E. coli
6His, N-terminus
21.4 kDa
10 µg/$251; 50 µg/$311; 100 µg/$402; 200 µg/$502; 1 mg/$1,365; 500 µg/$1,002; 5 mg/$2,254; 2 mg/$1,523
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APOB / Apolipoprotein B Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
APOB / Apolipoprotein B Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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APOB / Apolipoprotein B Goat anti-Human Polyclonal Antibody
Human
ELISA, Flo, ID, IHC, IHC-P, IP, WB
Unconjugated
0.5 mg/$460
APOB / Apolipoprotein B Antibody - Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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APOB / Apolipoprotein B Goat anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$495
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APOB / Apolipoprotein B Mouse anti-Human Monoclonal (APC) Antibody
Human
ICC, IP, WB
APC Conjugated
100 µl/$411; 200 µl/$497; 500 µl/$864; 1 ml/$1,025; 2 ml/$1,337; 5 ml/$2,507
Viewing 1-25 of 170 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).