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Catalog Number Size Price
LS-C775092-100 100 µg (1 mg/ml) $479 
Apg7 / ATG7 Antibody - Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Apg7 / ATG7 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.
Apg7 / ATG7 Antibody - Rabbit Anti-ATG7 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate
Apg7 / ATG7 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical cancer cell line (HeLa) lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.
Apg7 / ATG7 Antibody - Western blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.
Apg7 / ATG7 Antibody - Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody  at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.
Apg7 / ATG7 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.
Apg7 / ATG7 Antibody - Rabbit Anti-ATG7 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate
Apg7 / ATG7 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical cancer cell line (HeLa) lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.
Apg7 / ATG7 Antibody - Western blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.
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Polyclonal Rabbit anti‑Human Apg7 / ATG7 Antibody (APC, IHC, IF, WB) LS‑C775092

Polyclonal Rabbit anti‑Human Apg7 / ATG7 Antibody (APC, IHC, IF, WB) LS‑C775092

Antibody:
Apg7 / ATG7 Rabbit anti-Human Polyclonal (APC) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C775092-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Apg7 / ATG7 Rabbit anti-Human Polyclonal (APC) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
ATG7 antibody LS-C775092 is an APC-conjugated rabbit polyclonal antibody to ATG7 (Apg7) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human Apg7 / ATG7
Synonyms
ATG7 | Apg7 | APG7L | APG7-LIKE | Autophagy related 7 | Autophagy-related protein 7 | HAGP7 | GSA7
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Allophycocyanin. Also available conjugated with Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of Human ATG7
Specificity
Detects 77.9 kDa.
Applications
  • IHC (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Apg7 / ATG7
O95352 NM_006395 NP_006386.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 12/26/2024