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Catalog Number	Size	Price
LS-C156613-400	400 µl	$393

Apg7 / ATG7 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Apg7 / ATG7 Antibody - Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548).

Apg7 / ATG7 Antibody - APG7L Antibody (R509) western blot of HeLa cell line lysates (35 ug/lane). The APG7L antibody detected the APG7L protein (arrow).

Apg7 / ATG7 Antibody - The anti-APG7L antibody is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L(arrow) was detected using the purified antibody.

Apg7 / ATG7 Antibody - Western blot of anti-hAPG7L-R509 antibody in 293 cell line lysates transiently transfected with the ATG7 gene (2 ug/lane). hAPG7L-R509(arrow) was detected using the purified antibody.

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Polyclonal Rabbit anti‑Human Apg7 / ATG7 Antibody (aa494‑523, IHC, IF, WB) LS‑C156613

Name: Anti-Apg7 / ATG7 Antibody | Rabbit anti-Human Polyclonal IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C156613-400
Price: 393.0000 USD
Availability: InStock

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Antibody:

Apg7 / ATG7 Rabbit anti-Human Polyclonal (aa494-523) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$393

LS-C156613-400

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(800) 227-6666

For Research Use Only

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Overview

Antibody:

Apg7 / ATG7 Rabbit anti-Human Polyclonal (aa494-523) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

ATG7 antibody LS-C156613 is an unconjugated rabbit polyclonal antibody to ATG7 (Apg7) (aa494-523) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.

Target

Human Apg7 / ATG7

Synonyms

See All Apg7 / ATG7 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Predicted

Rat (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa494-523

Specificity

This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:200)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About Apg7 / ATG7

Uniprot: O95352 NCBI: NM_006395 NP_006386.1