Target
Human APG5 / ATG5
Synonyms
ATG5 | APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Synthetic peptide corresponding to amino acids at the N-Terminus of human Atg5.
Applications
- Immunofluorescence (10 µg/ml)
- Western blot (1 - 2 µg/ml)
Usage
Immunoblotting: use at 1-2ug/ml. A band of ~50kDa, corresponding to Atg12-Atg5 complex, is detected. Note: the predicted molecular weight of ATG5 is ~32kDa. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Reconstitution
PBS or medium which is identical to that used in the assay system.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee