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Catalog Number Size Price
LS-B2170-50 50 µg (1 mg/ml) $485 
ABCB11 / BSEP Antibody - Anti-ABCB11 / BSEP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ABCB11 / BSEP Antibody - WB staining of crude plasma membrane purified from human liver tissue collected by fine needle biopsy.
ABCB11 / BSEP Antibody - Western blot of crude membranes isolated from human liver (hL), human.
ABCB11 / BSEP Antibody - Anti-ABCB11 / BSEP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ABCB11 / BSEP Antibody - WB staining of crude plasma membrane purified from human liver tissue collected by fine needle biopsy.
ABCB11 / BSEP Antibody - Western blot of crude membranes isolated from human liver (hL), human.
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IHC‑plus™ Monoclonal Mouse anti‑Human ABCB11 / BSEP Antibody (aa705‑718, IHC, WB) LS‑B2170

IHC‑plus™ Monoclonal Mouse anti‑Human ABCB11 / BSEP Antibody (aa705‑718, IHC, WB) LS‑B2170

Note: This antibody replaces LS-C4240
Antibody:
ABCB11 / BSEP Mouse anti-Human Monoclonal (aa705-718) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B2170-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ABCB11 / BSEP Mouse anti-Human Monoclonal (aa705-718) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BSEP antibody LS-B2170 is an unconjugated mouse monoclonal antibody to human BSEP (ABCB11) (aa705-718). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ABCB11 / BSEP
Synonyms
ABCB11 | ABC16 | Bile salt export pump | BSEP | PGY4 | Sister p-glycoprotein | BRIC2 | PFIC-2 | PFIC2 | SPGP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa 705-718 (Y705LVHEPPLAVVDHK718) of human BSEP (bile salt export pump). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%); Marmoset, Dog (93%).
Epitope
aa705-718
Specificity
Recognizes human BSEP. Does not cross-react with human P-glycoprotein.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2170 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2170 was determined to be 5 ug/ml.Western Blot: 1:50-1:200Immunocytochemistry: Cytospin preparationsOptimal dilutions to be determined by the researcher.
Presentation
PBS pH 7.2, 0.7% BSA, 100mM sodium azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCB11 / BSEP
O95342 NM_003742 NP_003733.2

Validation

ABCB11 / BSEP Antibody - Anti-ABCB11 / BSEP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ABCB11 / BSEP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ABCB11 / BSEP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ABCB11 / BSEP Antibody (aa705-718) for IHC, ICC, WB/Western LS-B2170 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/13/2024