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Antibodies > AMIGO2 > LS-C677124
 
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Catalog Number Size Price
LS-C677124-50 50 µg $294 
LS-C677124-100 100 µg $360 
AMIGO2 Antibody - Immunohistochemistry Dilution at 1:800 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
AMIGO2 Antibody - Immunohistochemistry Dilution at 1:800 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
AMIGO2 Antibody - Immunofluorescence staining of A549 cells diluted at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
AMIGO2 Antibody - Western Blot Positive WB detected in:Rat liver tissue All Lanes:AMIGO2 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 KDa Observed band size: 58 KDa
AMIGO2 Antibody - Immunohistochemistry Dilution at 1:800 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
AMIGO2 Antibody - Immunohistochemistry Dilution at 1:800 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
AMIGO2 Antibody - Immunofluorescence staining of A549 cells diluted at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
AMIGO2 Antibody - Western Blot Positive WB detected in:Rat liver tissue All Lanes:AMIGO2 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 KDa Observed band size: 58 KDa
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Polyclonal Rabbit anti‑Human AMIGO2 Antibody (IHC, IF, WB) LS‑C677124

Polyclonal Rabbit anti‑Human AMIGO2 Antibody (IHC, IF, WB) LS‑C677124

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Antibody:
AMIGO2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin FITC HRP
Price
Catalog Number
$294
LS-C677124-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AMIGO2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin FITC HRP

Specifications

Description
AMIGO2 antibody LS-C677124 is an unconjugated rabbit polyclonal antibody to human AMIGO2. Validated for ELISA, IF, IHC and WB.
Target
Human AMIGO2
Synonyms
AMIGO2 | ALI1 | Alivin 1 | Alivin-1 | Alvin 1 | AMIGO-2 | Amphoterin induced gene 2 | Amphoterin-induced protein 2 | Transmembrane protein AMIGO2 | DEGA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human Amphoterin-induced protein 2 protein (420-522AA)
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AMIGO2
Q86SJ2 NM_181847 NP_862830.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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