Bacillus amyloliquefaciens Alpha Amylase

Rabbit

Bacillus amyloliquefaciens (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens a-Amylase protein.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens]. Cross reactivity against a-Amylase from other tissues and species is unknown.

• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

Alpha Amylase antibody has been assayed against 1.0 ug of Alpha Amylase [Bacillus amyloliquefaciens] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2000 to 1:10000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

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