LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > ALOX12 / 12 Lipoxygenase > LS-C680154
 
Print Page  
Catalog Number Size Price
LS-C680154-50 50 µg $294 
LS-C680154-100 100 µg $360 
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunofluorescence staining of A549 cells diluted at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ALOX12 / 12 Lipoxygenase Antibody - Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All Lanes: ALOX12 antibody at 3.53µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 KDa Observed band size: 76 KDa
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunofluorescence staining of A549 cells diluted at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ALOX12 / 12 Lipoxygenase Antibody - Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All Lanes: ALOX12 antibody at 3.53µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 KDa Observed band size: 76 KDa
1 of 4
2 of 4
3 of 4
4 of 4

Polyclonal Rabbit anti‑Human ALOX12 / 12 Lipoxygenase Antibody (IHC, IF, WB) LS‑C680154

Polyclonal Rabbit anti‑Human ALOX12 / 12 Lipoxygenase Antibody (IHC, IF, WB) LS‑C680154

Submit Review LSBio Guarantee
Antibody:
ALOX12 / 12 Lipoxygenase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin HRP FITC
Price
Catalog Number
$294
LS-C680154-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ALOX12 / 12 Lipoxygenase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin HRP FITC

Specifications

Description
12 Lipoxygenase antibody LS-C680154 is an unconjugated rabbit polyclonal antibody to human 12 Lipoxygenase (ALOX12). Validated for ELISA, IF, IHC and WB.
Target
Human ALOX12 / 12 Lipoxygenase
Synonyms
ALOX12 | 12S-lipoxygenase | 12-lipoxygenase | 12S-LOX | 12(S)-lipoxygenase | 12-LOX | 12 Lipoxygenase | Arachidonate 12-lipoxygenase | LOG12 | Platelet 12-LOX | Platelet-type 12-lipoxygenase | Platelet-type lipoxygenase 12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Arachidonate 12-lipoxygenase, 12S-type protein (508-658AA)
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALOX12 / 12 Lipoxygenase
P18054 NM_000697 NP_000688.2

Publications (0)

Submit a Publication for LS-C680154

Customer Reviews (0)

Submit a review for LS-C680154

Featured Products

ALOX12 / 12 Lipoxygenase Antibody - Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ALOX12 / 12 Lipoxygenase Antibody (aa578‑627) LS‑C81851
Species: Human
Applications: Western blot
ALOX12 / 12 Lipoxygenase Antibody - Anti-ALOX12/12 Lipoxygenase/12-LOX antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ ALOX12 / 12 Lipoxygenase Antibody (aa618‑650) LS‑B10714
Species: Human
Applications: IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product
ALOX12 / 12 Lipoxygenase Antibody LS‑C306215
Species: Human
Applications: Immunofluorescence, Western blot
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
ALOX12 / 12 Lipoxygenase Antibody (clone OTI1B2) LS‑C794119
Species: Human
Applications: IHC
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry of paraffin-embedded Mouse brain using ALOX12 Polyclonal Antibody at dilution of 1:100 (40x lens).
ALOX12 / 12 Lipoxygenase Antibody LS‑C833341
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence
Product
ALOX12 / 12 Lipoxygenase Antibody LS‑C749685
Species: Human, Mouse, Rat
Applications: IHC
See all ALOX12 / 12 Lipoxygenase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com