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Antibodies > ALOX12 / 12 Lipoxygenase > LS-C680154
 
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Catalog Number Size Price
LS-C680154-50 50 µg $294 
LS-C680154-100 100 µg $360 
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunofluorescence staining of A549 cells diluted at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ALOX12 / 12 Lipoxygenase Antibody - Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All Lanes: ALOX12 antibody at 3.53µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 KDa Observed band size: 76 KDa
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ALOX12 / 12 Lipoxygenase Antibody - Immunofluorescence staining of A549 cells diluted at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ALOX12 / 12 Lipoxygenase Antibody - Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All Lanes: ALOX12 antibody at 3.53µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 KDa Observed band size: 76 KDa
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Polyclonal Rabbit anti‑Human ALOX12 / 12 Lipoxygenase Antibody (IHC, IF, WB) LS‑C680154

Polyclonal Rabbit anti‑Human ALOX12 / 12 Lipoxygenase Antibody (IHC, IF, WB) LS‑C680154

Submit Review LSBio Guarantee
Antibody:
ALOX12 / 12 Lipoxygenase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin HRP FITC
Price
Catalog Number
$294
LS-C680154-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ALOX12 / 12 Lipoxygenase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Biotin HRP FITC

Specifications

Description
12 Lipoxygenase antibody LS-C680154 is an unconjugated rabbit polyclonal antibody to human 12 Lipoxygenase (ALOX12). Validated for ELISA, IF, IHC and WB.
Target
Human ALOX12 / 12 Lipoxygenase
Synonyms
ALOX12 | 12S-lipoxygenase | 12-lipoxygenase | 12S-LOX | 12(S)-lipoxygenase | 12-LOX | 12 Lipoxygenase | Arachidonate 12-lipoxygenase | LOG12 | Platelet 12-LOX | Platelet-type 12-lipoxygenase | Platelet-type lipoxygenase 12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Arachidonate 12-lipoxygenase, 12S-type protein (508-658AA)
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALOX12 / 12 Lipoxygenase
P18054 NM_000697 NP_000688.2

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IHC‑plus™ ALOX12 / 12 Lipoxygenase Antibody (aa618‑650) LS‑B10714
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Applications: IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
ALOX12 / 12 Lipoxygenase Antibody - Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ALOX12 / 12 Lipoxygenase Antibody (aa578‑627) LS‑C81851
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ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemistry of paraffin-embedded Mouse brain using ALOX12 Polyclonal Antibody at dilution of 1:100 (40x lens).
ALOX12 / 12 Lipoxygenase Antibody LS‑C833341
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence
ALOX12 / 12 Lipoxygenase Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
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See all ALOX12 / 12 Lipoxygenase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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