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Antibodies > AIFM1 / AIF / PDCD8 > LS-C156184
 
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Catalog Number Size Price
LS-C156184-400 400 µl $393 
AIFM1 / AIF / PDCD8 Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AIFM1 / AIF / PDCD8 Antibody - Fluorescent confocal image of U251 cells stained with AIFM1 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated AIFM1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 immunoreactivity is localized to the cytoplasm of U251 cells.
AIFM1 / AIF / PDCD8 Antibody - Fluorescent image of U251 cells stained with AIFM1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated AIFM1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 immunoreactivity is localized to the cytoplasm of U251 cells.
AIFM1 / AIF / PDCD8 Antibody - Western blot of AIFM1 Antibody in 293 cell line lysates (35 ug/lane). AIFM1 (arrow) was detected using the purified antibody.
AIFM1 / AIF / PDCD8 Antibody - AIFM1 Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AIFM1 / AIF / PDCD8 Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AIFM1 / AIF / PDCD8 Antibody - Fluorescent confocal image of U251 cells stained with AIFM1 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated AIFM1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 immunoreactivity is localized to the cytoplasm of U251 cells.
AIFM1 / AIF / PDCD8 Antibody - Fluorescent image of U251 cells stained with AIFM1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated AIFM1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 immunoreactivity is localized to the cytoplasm of U251 cells.
AIFM1 / AIF / PDCD8 Antibody - Western blot of AIFM1 Antibody in 293 cell line lysates (35 ug/lane). AIFM1 (arrow) was detected using the purified antibody.
AIFM1 / AIF / PDCD8 Antibody - AIFM1 Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human AIFM1 / AIF / PDCD8 Antibody (aa70‑98, IHC, IF, WB) LS‑C156184

Polyclonal Rabbit anti‑Human AIFM1 / AIF / PDCD8 Antibody (aa70‑98, IHC, IF, WB) LS‑C156184

Submit Review LSBio Guarantee
Antibody:
AIFM1 / AIF / PDCD8 Rabbit anti-Human Polyclonal (aa70-98) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C156184-400
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AIFM1 / AIF / PDCD8 Rabbit anti-Human Polyclonal (aa70-98) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PDCD8 antibody LS-C156184 is an unconjugated rabbit polyclonal antibody to human PDCD8 (AIFM1 / AIF) (aa70-98). Validated for Flow, IF, IHC and WB.
Target
Human AIFM1 / AIF / PDCD8
Synonyms
AIFM1 | AIF | COXPD6 | PDCD8 | Programmed cell death 8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa70-98
Specificity
This AIFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human AIFM1.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AIFM1 / AIF / PDCD8
O95831 NM_004208 NP_004199.1

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Featured Products

AIFM1 / AIF / PDCD8 Antibody - IHC of AIF in formalin-fixed, paraffin-embedded normal human colon using AIFM1 / AIF / PDCD8 Antibody at 1:2000. Hematoxylin-Eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.
AIFM1 / AIF / PDCD8 Antibody (aa151‑169) LS‑C147989
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
AIFM1 / AIF / PDCD8 Antibody - Anti-AIFM1 / AIF antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
IHC‑plus™ AIFM1 / AIF / PDCD8 Antibody LS‑B7901
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
AIFM1 / AIF / PDCD8 Antibody - Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AIFM1 / AIF / PDCD8 Antibody (aa211‑260) LS‑B1508
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
AIFM1 / AIF / PDCD8 Antibody - Anti-AIFM1 / AIF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ AIFM1 / AIF / PDCD8 Antibody LS‑B3765
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot, ELISA
AIFM1 / AIF / PDCD8 Antibody - Immunohistochemical analysis of AIFM1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AIFM1 / AIF / PDCD8 Antibody (N‑Terminus) LS‑C353147
Species: Human, Mouse, Rat, Sheep
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
See all AIFM1 / AIF / PDCD8 Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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