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Catalog Number Size Price
LS-C20327-50 50 µl $491 
Antibody

Polyclonal Rabbit anti‑Rat ADRA2A Antibody (IHC, IF) LS‑C20327

Polyclonal Rabbit anti‑Rat ADRA2A Antibody (IHC, IF) LS‑C20327

Antibody:
ADRA2A Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, ICC, IF
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$491
LS-C20327-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ADRA2A Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, ICC, IF
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ADRA2A antibody LS-C20327 is an unconjugated rabbit polyclonal antibody to rat ADRA2A. Validated for ICC, IF and IHC.
Target
Rat ADRA2A
Synonyms
ADRA2A | Alpha-2A adrenoreceptor | Alpha-2AAR | Alpha-2AAR subtype C10 | ADRA2 | ADRA2R | ADRAR | Adrenoceptor alpha 2A | Alpha 2a | Alpha(2A)-AR | Alpha2a | ALPHA2AAR | Alpha-2A adrenoceptor | Adrenergic alpha 2a receptor | Adrenergic alpha 2a receptor | Alpha-2A adrenergic receptor
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
(C)KASRWRGRGNREKR; Cysteine added for conjugation. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Panda, Cat, Bovine, Pig, Opossum, Guinea pig, Lizard (93%); Pufferfish, Zebrafish (86%).
Specificity
Recognizes rat alpha 2a Adrenergic Receptor. Shows positive staining in rat spinal cord dorsal horn, ventral horn motor neurons and retina. When examined in other brain regions known to express alpha 2a, staining was negative. Species cross-reactivity: Mouse and human.
Applications
  • IHC (1:100 - 1:500)
  • ICC
  • Immunofluorescence
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Immunohistochemistry and Immunocytochemistry. Immunohistochemistry: 1:100-1:500; Antiserum was used on perfusion fixed tissue. Perfusion: 1) Calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and cover-slipped. Staining was examined using fluorescence microscopy.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADRA2A
P08913 NM_000681 NP_000672.3

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Requested From: United States
Date Requested: 11/22/2024