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Antibodies > ADGRE5 / CD97 > LS-C675303
 
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Catalog Number Size Price
LS-C675303-50 50 µl $316 
LS-C675303-100 100 µl $386 
ADGRE5 / CD97 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ADGRE5 / CD97 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ADGRE5 / CD97 Antibody - Immunofluorescence staining of Hela cells diluted at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ADGRE5 / CD97 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ADGRE5 / CD97 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ADGRE5 / CD97 Antibody - Immunofluorescence staining of Hela cells diluted at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Monoclonal Rabbit anti‑Human ADGRE5 / CD97 Antibody (IHC, IF) LS‑C675303

Monoclonal Rabbit anti‑Human ADGRE5 / CD97 Antibody (IHC, IF) LS‑C675303

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Antibody:
ADGRE5 / CD97 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675303-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ADGRE5 / CD97 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD97 antibody LS-C675303 is an unconjugated rabbit monoclonal antibody to human CD97 (ADGRE5). Validated for ELISA, IF and IHC.
Target
Human ADGRE5 / CD97
Synonyms
ADGRE5 | CD97 | CD97 molecule | Leukocyte antigen CD97 | TM7LN1 | CD97 antigen
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRE5 / CD97
P48960 NM_078481 NP_510966.1

Publications (0)

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Customer Reviews (0)

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ADGRE5 / CD97 Antibody - Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ ADGRE5 / CD97 Antibody (aa220‑270) LS‑B1780
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Applications: IHC, IHC - Paraffin, Western blot
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Species: Human, Monkey, Dog
Applications: IHC, IHC - Paraffin, ICC
ADGRE5 / CD97 Antibody - Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
ADGRE5 / CD97 Antibody LS‑C18770
Species: Mouse, Human, Chimpanzee
Applications: Immunofluorescence, Western blot, ELISA
Product
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Species: Human
Applications: ICC, Immunofluorescence, Flow Cytometry, ELISA
ADGRE5 / CD97 Antibody - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD97 mouse mAb with DAB staining.
ADGRE5 / CD97 Antibody (clone 6D7E6) LS‑C828393
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Human EPO / Erythropoietin Protein (Recombinant His-GST) (aa28-193) - LS-G11249
Source: E. coli
Tag: His-GST
See all ADGRE5 / CD97 Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/16/2024
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