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Antibodies > ACTN1 > LS-C807276
 
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Catalog Number Size Price
LS-C807276-100 100 µl (1 mg/ml) $379 
LS-C807276-200 200 µl (1 mg/ml) $421 
ACTN1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ACTN1 Antibody - Western blot analysis of ACTN1/2/3/4 using COLO205 whole cells lysates
ACTN1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ACTN1 Antibody - Western blot analysis of ACTN1/2/3/4 using COLO205 whole cells lysates
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Polyclonal Rabbit anti‑Human ACTN1 Antibody (IF, WB) LS‑C807276

Polyclonal Rabbit anti‑Human ACTN1 Antibody (IF, WB) LS‑C807276

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Antibody:
ACTN1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C807276-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ACTN1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ACTN1 antibody LS-C807276 is an unconjugated rabbit polyclonal antibody to ACTN1 from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human ACTN1
Synonyms
ACTN1 | Alpha-actinin-1 | Actinin 1 smooth muscle | Actinin, alpha 1 | F-actin cross-linking protein | Non-muscle alpha-actinin-1 | ASMA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ACTN1/2/3/4.
Specificity
ACTN1/2/3/4 Antibody detects endogenous levels of total ACTN1/2/3/4.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTN1
P12814 NM_001102 NP_001093.1

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ACTN1 Antibody - ICC/IF analysis of ACTN1 in Balb-3T3 cells. The cell was stained with ACTN1 antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Reactivity: Human
Range: 0.156-10 ng/ml
ACTN1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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See all ACTN1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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