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Catalog Number Size Price
LS-C287370-10 10 µl $295 
LS-C287370-100 100 µl (0.2 mg/ml) $514 
ACTG1 / Gamma Actin Antibody - Detection of Human Actin by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
ACTG1 / Gamma Actin Antibody - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ACTG1 / Gamma Actin Antibody - Detection of Human and Mouse Actin by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-Actin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ACTG1 / Gamma Actin Antibody - Detection of Human Actin by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
ACTG1 / Gamma Actin Antibody - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ACTG1 / Gamma Actin Antibody - Detection of Human and Mouse Actin by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-Actin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Polyclonal Rabbit anti‑Human ACTG1 / Gamma Actin Antibody (N‑Terminus, IHC, WB) LS‑C287370

Polyclonal Rabbit anti‑Human ACTG1 / Gamma Actin Antibody (N‑Terminus, IHC, WB) LS‑C287370

Antibody:
ACTG1 / Gamma Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287370-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACTG1 / Gamma Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Gamma Actin antibody LS-C287370 is an unconjugated rabbit polyclonal antibody to Gamma Actin (ACTG1) (N-Terminus) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human ACTG1 / Gamma Actin
Synonyms
ACTG1 | ACTG | Actin, gamma 1 | BRWS2 | Cytoskeletal gamma-actin | DFNA20 | Actin, cytoplasmic 2 | Gamma-actin | DFNA26
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Xenopus laevis (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 71 corresponding to amino acids near the N-Terminus of UniprotID: P63261
Epitope
N-Terminus
Specificity
The epitope recognized by A300-485A maps to the N-terminus of human Gamma-actin using the numbering given in Swiss-Prot entry P63261 (GeneID 71).
Applications
  • IHC (1:2000 - 1:10000)
  • Western blot (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Immunoprecipitation
Usage
IHC: Epitope retrieval may not be necessary. In some instances epitope retrieval with citrate buffer pH 6.0 may enhance staining for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTG1 / Gamma Actin
P63261 NM_001614 NP_001605.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024