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Antibodies > ACTA1 / Skeletal Muscle Actin > LS-C675296
 
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Catalog Number Size Price
LS-C675296-50 50 µl $316 
LS-C675296-100 100 µl $386 
ACTA1 / Skeletal Muscle Actin Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ACTA1 / Skeletal Muscle Actin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ACTA1 / Skeletal Muscle Actin Antibody - Western Blot Positive WB detected in:Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue All Lanes:Actin antibody at 0.95µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa
ACTA1 / Skeletal Muscle Actin Antibody - Overlay histogram showing Hela cells stained with ACTA1 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
ACTA1 / Skeletal Muscle Actin Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
ACTA1 / Skeletal Muscle Actin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ACTA1 / Skeletal Muscle Actin Antibody - Western Blot Positive WB detected in:Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue All Lanes:Actin antibody at 0.95µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa
ACTA1 / Skeletal Muscle Actin Antibody - Overlay histogram showing Hela cells stained with ACTA1 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Monoclonal Rabbit anti‑Human ACTA1 / Skeletal Muscle Actin Antibody (IHC, IF, WB) LS‑C675296

Monoclonal Rabbit anti‑Human ACTA1 / Skeletal Muscle Actin Antibody (IHC, IF, WB) LS‑C675296

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Antibody:
ACTA1 / Skeletal Muscle Actin Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675296-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ACTA1 / Skeletal Muscle Actin Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Skeletal Muscle Actin antibody LS-C675296 is an unconjugated rabbit monoclonal antibody to human Skeletal Muscle Actin (ACTA1). Validated for ELISA, Flow, IF, IHC and WB.
Target
Human ACTA1 / Skeletal Muscle Actin
Synonyms
ACTA1 | ACTA | Actin, alpha skeletal muscle | Alpha-actin-1 | CFTD | CFTD1 | CFTDM | MPFD | NEM1 | NEM2 | ASMA | NEM3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTA1 / Skeletal Muscle Actin
P68133 NM_001100 NP_001091.1

Publications (0)

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ACTA1 / Skeletal Muscle Actin Antibody - Immunohistochemistry analysis of paraffin-embedded human muscle tissue, using Actin-alpha-1 Antibody. The picture on the right is blocked with the synthesized peptide.
ACTA1 / Skeletal Muscle Actin Antibody (aa1‑50) LS‑C118384
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
See all ACTA1 / Skeletal Muscle Actin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

Orders:
customerservice@vectorlabs.com

For technical assistance:
Technical.Support@LSBio.com