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| Catalog Number | Size | Price |
|---|---|---|
| LS-C134870-1 | 1 ml | $522 |
Monoclonal Mouse anti‑Human ABCC2 / MRP2 Antibody (clone M2III‑5, Concentrated, IHC, WB) LS‑C134870
Monoclonal Mouse anti‑Human ABCC2 / MRP2 Antibody (clone M2III‑5, Concentrated, IHC, WB) LS‑C134870
Antibody:
ABCC2 / MRP2 Mouse anti-Human Monoclonal (Concentrated) (M2III-5) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Concentrated
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ABCC2 / MRP2 Mouse anti-Human Monoclonal (Concentrated) (M2III-5) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Concentrated
Specifications
Description
MRP2 antibody LS-C134870 is an unconjugated mouse monoclonal antibody to human MRP2 (ABCC2). Validated for Flow, ICC, IHC and WB.
Target
Human ABCC2 / MRP2
Synonyms
ABCC2 | ABC30 | CMOAT1 | CMOAT | DJS | Multidrug resistance protein 2 | MRP2 | CMRP
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
M2III-5
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Concentrated
Immunogen
Fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein.
Specificity
M2III-5 reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2III-5 was raised against a fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein. The Mab detects rat, mouse and human MRP2. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 orMRP5 gene products.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- ICC
- Western blot
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
M2III-5 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dilution. on acetone fixed cytospin preparations. For immunohistochemistry: M2III-5 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG (1:25, Dako) and a monoclonal mouse APAAP complex (1:50, Dako). M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 5 min 3 times. After incubation of M2III-5 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution, and anti-mouse-HRP.
Presentation
Tissue Culture Supernatant, 0.1% Sodium Azide, 0.7% BSA, Serum-free
Storage
Store at 2-8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ABCC2 / MRP2
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Featured Products
Species:
Human
Applications:
Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Rat, Human
Applications:
IHC, IHC - Frozen, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
