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Catalog Number Size Price
LS-B3454-200 200 µl $460 
ABCB5 Antibody - Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ABCB5 Antibody - Western blot of ABCB5 Antibody in A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified antibody.
ABCB5 Antibody - Flow Cytometry using ABCB5 Antibody on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
ABCB5 Antibody - Flow Cytometry using ABCB5 Antibody on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
ABCB5 Antibody - Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ABCB5 Antibody - Western blot of ABCB5 Antibody in A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified antibody.
ABCB5 Antibody - Flow Cytometry using ABCB5 Antibody on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
ABCB5 Antibody - Flow Cytometry using ABCB5 Antibody on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
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IHC‑plus™ Polyclonal Rabbit anti‑Human ABCB5 Antibody (aa1‑30, IHC, WB) LS‑B3454

IHC‑plus™ Polyclonal Rabbit anti‑Human ABCB5 Antibody (aa1‑30, IHC, WB) LS‑B3454

Note: This antibody replaces LS-C100370
Antibody:
ABCB5 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3454-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ABCB5 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ABCB5 antibody LS-B3454 is an unconjugated rabbit polyclonal antibody to human ABCB5 (aa1-30). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human ABCB5
Synonyms
ABCB5 | ABCB5alpha | ABCB5 P-gp | ABCB5beta | ATP-binding cassette protein | EST422562 | P-glycoprotein ABCB5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa1-30
Specificity
This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3454 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3454 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCB5
Q2M3G0 NM_178559 NP_848654.3

Validation

ABCB5 Antibody - Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ ABCB5 Antibody (aa1-30) for IHC, WB/Western, Flow LS-B3454 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells. Freeman JB, Gray ES, Millward M, Pearce R, Ziman M. Journal of translational medicine. 2012 10:192.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/22/2024