Cucurbita AAO / L-Ascorbate Oxidase

Rabbit

Cucurbita (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Ascorbate Oxidase [cucurbita].

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined.

• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:2500 - 1:15000)

Anti-Ascorbate Oxidase has been assayed against 1.0 ug of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2000 to 1:10000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: P24792