Target
Cucurbita AAO / L-Ascorbate Oxidase
Reactivity
Cucurbita
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
Ascorbate Oxidase [cucurbita].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources is unknown.
Applications
- Western blot (1:2000 - 1:10000)
- Immunoprecipitation (1:100)
- ELISA (1:25000 - 1:125000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AAO / L-Ascorbate Oxidase