Rat A2M / Alpha-2-Macroglobulin

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat alpha-2 macroglobulin.

• Immunodiffusion

The reactivity of the antiserum is restricted to rat alpha-2 macroglobulin. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 macroglobulin. No reaction is obtained with any other plasma protein components or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of alpha-2 macroglobulin in rat plasma or other body fluids or to determine its concentration. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays, but this does exclude such use if proper controls are included.

Delipidated, heat-inactivated, lyophilized antiserum. No preservative.

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01023 NCBI: NM_000014 NP_000005.2