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Catalog Number Size Price
LS-C744671-25 25 µl (1 mg/ml) $304 
A2M / Alpha-2-Macroglobulin Antibody - Western Blot of Goat Anti-Alpha-2-Macroglobulin antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
A2M / Alpha-2-Macroglobulin Antibody - Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
A2M / Alpha-2-Macroglobulin Antibody - Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
A2M / Alpha-2-Macroglobulin Antibody - Western Blot of Goat Anti-Alpha-2-Macroglobulin antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
A2M / Alpha-2-Macroglobulin Antibody - Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
A2M / Alpha-2-Macroglobulin Antibody - Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
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Polyclonal Goat anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (Liquid, IHC, WB) LS‑C744671

Polyclonal Goat anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (Liquid, IHC, WB) LS‑C744671

Antibody:
A2M / Alpha-2-Macroglobulin Goat anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C744671-25
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
A2M / Alpha-2-Macroglobulin Goat anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid

Specifications

Description
Alpha-2-Macroglobulin antibody LS-C744671 is an unconjugated goat polyclonal antibody to human Alpha-2-Macroglobulin (A2M). Validated for ELISA, IHC and WB.
Target
Human A2M / Alpha-2-Macroglobulin
Synonyms
A2M | CPAMD5 | A2MD | FWP007 | Alpha-2-M | Alpha-2-macroglobulin | S863-7
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
A2-Macroglobulin [Human Plasma]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma]. Cross reactivity against a2-Macroglobulin from other sources is unknown.
Applications
  • IHC
  • Western blot (1:2000 - 1:20000)
  • ELISA (1:80000 - 1:400000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About A2M / Alpha-2-Macroglobulin
P01023 NM_000014 NP_000005.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024