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Catalog Number	Size	Price
LS-C355390-1	1 mg	$857

A2M / Alpha-2-Macroglobulin Antibody - Western blot analysis of Alpha2-Macroglobulin antibody was performed by loading 1000ng, 500ng, 250ng and 125ng of a Human Alpha2-Macroglobulin Recombinant protein and 5ul PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% BSA in TBST for at least 1 hour at room temperature. Alpha 2 Macroglobulin was detected at ~180kD using an Alpha2-Macroglobulin monoclonal antibody at a concentration of 2 µg/mL in 5% BSA in TBST overnight at 4C on a rocking platform, followed by an incubation with a goat anti-mouse IgG-HRP Superclonal secondary antibody at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

A2M / Alpha-2-Macroglobulin Antibody - Western blot analysis of Alpha2-Macroglobulin antibody was performed by loading 5ul of Human Plasma and 5ul PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% milk in TBST for at least 1 hour at room temperature. Alpha 2 Macroglobulin was detected at ~180kD using an Alpha2-Macroglobulin monoclonal antibody at a concentration of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by an incubation with a goat anti-mouse IgG-HRP Superclonal secondary antibody at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.

A2M / Alpha-2-Macroglobulin Antibody - Direct ELISA analysis of Alpha2-Macroglobulin was performed by coating wells of a 96-well plate with 100ul per well of Alpha2- Macroglobulin recombinant protein diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse Alpha2-Macroglobulin monoclonal antibody starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 0.03125 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid and absorbances were read on a spectrophotometer at 450-550 nm.

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Monoclonal Mouse anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (clone F1‑P1C11 #3, WB) LS‑C355390

Name: Anti-A2M / Alpha-2-Macroglobulin Antibody | Mouse anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C355390-1
Price: 857.0000 USD
Availability: InStock

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Antibody:

A2M / Alpha-2-Macroglobulin Mouse anti-Human Monoclonal (F1-P1C11 #3) Antibody

Application:

WB, IP, ELISA, RIA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$857

LS-C355390-1

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Overview

Antibody:

A2M / Alpha-2-Macroglobulin Mouse anti-Human Monoclonal (F1-P1C11 #3) Antibody

Application:

WB, IP, ELISA, RIA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Alpha-2-Macroglobulin antibody LS-C355390 is an unconjugated mouse monoclonal antibody to human Alpha-2-Macroglobulin (A2M). Validated for ELISA, IP, RIA and WB.

Target

Human A2M / Alpha-2-Macroglobulin

Synonyms

A2M | CPAMD5 | A2MD | FWP007 | Alpha-2-M | Alpha-2-macroglobulin | S863-7

See All A2M / Alpha-2-Macroglobulin Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Clone

F1-P1C11 #3

Conjugations

Unconjugated

Purification

Ion exchange chromatography

Modifications

Unmodified

Immunogen

Purified human plasma AMG.

Specificity

The antibody targets Alpha2-Macroglobulin in ELISA, immunoprecipitation, and radioimmunoassay applications and shows reactivity with Human samples. It detects Alpha2-Macroglobulin which has a predicted molecular weight of approximately 162 kD.

Applications