ZAP70

zeta-chain (TCR) associated protein kinase 70kDa

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

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Products

ELISA Kits (6)

Type

Cell-Based Phosphorylation (2)

Custom (2)

Sandwich (2)

Target

ZAP70 (6)

Reactivity

Human (5)

Mouse (3)

Rat (1)

Format

96-Well Microplate (3)

96-Well Strip Plate (3)

Publications

No (6)

Sample Type

Adherent Cell Cultures (2)

Plasma (2)

Serum (2)

Tissue Homogenates (1)

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Mouse/Human Phospho-ZAP70 (Tyr315) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1238

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

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Mouse/Human/Rat Phospho-ZAP70 (Tyr493) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1650

Type:

Cell-Based Phosphorylation

Format:

96-Well Microplate

Reactivity:

Mouse, Human, Rat

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$668

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Human ZAP70 (Sandwich ELISA) ELISA Kit - LS-F33470

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.312 - 20 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

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Human ZAP70 (Sandwich ELISA) ELISA Kit - LS-F12991

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.312 - 20 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$669

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Mouse ZAP70 (Custom ELISA) ELISA Kit - LS-F20137

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Mouse

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$669

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Human ZAP70 (Custom ELISA) ELISA Kit - LS-F50766

Type:

Custom

Format:

96-Well Microplate

Reactivity:

Human

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$610

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