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Vimentin

Vimentin is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Gene Name: Vimentin
Synonyms: VIM, Vimentin, vimenin
Target Sequences: NM_003380 NP_003371.2 P08670

Publications (11)

1
Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor. Rankin EB, Tomaszewski JE, Haase VH. Cancer research. 2006 66:2576-83. [PubMed:16510575] [PMC:PMC3514875]
2
The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation. Okawa T, Michaylira CZ, Kalabis J, Stairs DB, Nakagawa H, Andl CD, Johnstone CN, Klein-Szanto AJ, El-Deiry WS, Cukierman E, Herlyn M, Rustgi AK. Genes & development. 2007 21:2788-803. [PubMed:17974918] [PMC:PMC2045132]
3
Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation. Johnstone CN, Mongroo PS, Rich AS, Schupp M, Bowser MJ, Delemos AS, Tobias JW, Liu Y, Hannigan GE, Rustgi AK. Molecular and cellular biology. 2008 28:687-704. [PubMed:17998334] [PMC:PMC2223422]
4
Met induces mammary tumors with diverse histologies and is associated with poor outcome and human basal breast cancer. Ponzo MG, Lesurf R, Petkiewicz S, O'Malley FP, Pinnaduwage D, Andrulis IL, Bull SB, Chughtai N, Zuo D, Souleimanova M, Germain D, Omeroglu A, Cardiff RD, Hallett M, Park M. Proceedings of the National Academy of Sciences of the United States of America. 2009 106:12903-8. [PubMed:19617568] [PMC:PMC2722321]
5
Genomic analysis of reactive astrogliosis. Zamanian JL, Xu L, Foo LC, Nouri N, Zhou L, Giffard RG, Barres BA. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:6391-410. (ICC, IHC; Mouse) [PubMed:22553043] [PMC:PMC3480225]
6
Esophageal epithelial and mesenchymal cross-talk leads to features of epithelial to mesenchymal transition in vitro. Muir AB, Lim DM, Benitez AJ, Modayur Chandramouleeswaran P, Lee AJ, Ruchelli ED, Spergel JM, Wang ML. Experimental cell research. 2013 319:850-9. (ICC, IHC; Human) [PubMed:23237990] [PMC:PMC3593809]
7
Autocrine IL-8 promotes F-actin polymerization and mediate mesenchymal transition via ELMO1-NF-?B-Snail signaling in glioma. Zhang B, Shi L, Lu S, Sun X, Liu Y, Li H, Wang X, Zhao C, Zhang H, Wang Y. Cancer biology & therapy. 2015 16:898-911. (IHC, WB; Human) [Full Text Article] [PubMed:25870011] [PMC:PMC4623112] Related Antibodies: LS-C92412.
8
Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis. Planells-Palop V, Hazazi A, Feichtinger J, Jezkova J, Thallinger G, Alsiwiehri NO, Almutairi M, Parry L, Wakeman JA, McFarlane RJ. Molecular cancer. 2017 16:84. (ICC, IHC-P; Human) [Full Text Article] [PubMed:28446200] [PMC:PMC5406905] Related Antibodies: LS-B7191.
9
Ductal tree ablation by local delivery of ethanol prevents tumor formation in an aggressive mouse model of breast cancer. Elizabeth Kenyon, Jennifer J Westerhuis, Maximilian Volk, Jeremy Hix, Shatadru Chakravarty, Ethan Claucherty, Erin Zaluzec, Lisa Ramsey, Zach Madaj, Galen Hostetter, Bryn Eagleson, Erik Shapiro, Anna Moore, Lorenzo F Sempere. Breast cancer research : BCR. 2019 November;21:129. [Full Text Article] [PubMed:31779648] [PMC:PMC6883550] Related Antibodies: LS-B291.
10
Ablation of miR-144 increases vimentin expression and atherosclerotic plaque formation. Quan He, Fangfei Wang, Takashi Honda, Kenneth D Greis, Andrew N Redington. Scientific reports. 2020 Apr;10:6127. [Full Text Article] [PubMed:32273567] [PMC:PMC7145828]
11
Decrease of hepatic stellate cells in rats with enhanced sensitivity to clofibrate-induced hepatocarcinogenesis. Yamana D, Shimizu T, Fan Y, Miura T, Nanashima N, Yamada T, Hakamada K, Tsuchida S. Cancer science. 2011 Apr;102:735-41. [Full Text Article] [PubMed:21214675] Related Antibodies: LS-C40195.
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Products
Proteins (12)
Over-Expression Lysate (2)
Recombinant (10)
Vimentin (12)
SDS-PAGE (1)
No (12)
6His, C-terminus (1)
6His, N-terminus (2)
GST (1)
GST, N-terminus (1)
His (1)
His, N-Terminal (2)
His,C-terminus (1)
Myc-DDK (Flag) (3)
Human (10)
Mouse (1)
293T Cells (1)
Baculovirus (1)
E. coli (5)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Yeast (1)
Purified (4)
Low endotoxin level (2)
Vimentin Protein - Recombinant Vimentin (VIM) by SDS-PAGE
Select
E. coli
His, N-Terminal
55.1kDa
10 µg/$282; 50 µg/$450; 200 µg/$897; 100 µg/$717; 1 mg/$2,010; 500 µg/$1,547; 5 mg/$3,137; 2 mg/$2,198
Select
E. coli
6His, C-terminus
Less than 0.1 ng/µg of protein (less than 1EU/µg).
54.5 kDa
20 µg/$268; 100 µg/$349
Select
E. coli
His
57.6 kD
100 µg/$420; 1 mg/$1,355; 20 µg/$323
Select
293T Cells
Myc-DDK (Flag)
53.5 kDa
20 µg/$150
Select
Yeast
6His, N-terminus
55.5 kD
100 µg/$446; 20 µg/$336; 1 mg/$1,460
Vimentin Protein
Select
E. coli
His, N-Terminal
55 kDa
10 µg/$255; 50 µg/$323; 100 µg/$450; 200 µg/$562; 1 mg/$1,480; 500 µg/$1,096; 5 mg/$2,395; 2 mg/$1,646
Vimentin Protein - 12.5% SDS-PAGE of human VIM stained with Coomassie Blue
Select
Wheat Germ Extract
GST
80.1 kDa
10 µg/$479; 25 µg/$670
Vimentin Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Select
E. coli
6His, N-terminus
56 kDa
10 µg/$265; 50 µg/$315; 100 µg/$365
Vimentin Protein
Select
Baculovirus
His,C-terminus
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The secreted recombinant human VIM consists of 476 amino acids and predicts a molecular mass of 55 kDa. The apparent molecular mass of the protein is approximately 56 kDa in SDS-PAGE under reducing conditions due to glycosylation.
100 µg/$403; 1 mg/$1,697
Vimentin Protein - Purified recombinant protein VIM was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
53.5 kDa
20 µg/$1,107
Vimentin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
53.5 kDa
100 µg/$494
Viewing 1-12 of 12 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).