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UBE2F

ubiquitin-conjugating enzyme E2F (putative)

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Gene Name: ubiquitin-conjugating enzyme E2F (putative)
Synonyms: UBE2F, NCE2, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F
Target Sequences: NP_542409.1 Q969M7

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Products
Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
UBE2F (7)
No (7)
GST (1)
GST, N-terminus (1)
His (1)
His,N-terminus (1)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (3)
UBE2F Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
UBE2F Protein - 12.5% SDS-PAGE of human UBE2F stained with Coomassie Blue
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Wheat Germ Extract
GST
47.5 kDa
10 µg/$479; 25 µg/$670
UBE2F Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
20.9 kDa
20 µg/$150
UBE2F Protein - Purified recombinant protein UBE2F was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
20.9 kDa
20 µg/$1,107
UBE2F Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
20.9 kDa
100 µg/$494
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E. coli
His,N-terminus
22.9 kDa
100 µg/$661
UBE2F Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
GST, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
Viewing 1-7 of 7 product results


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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).