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TWF1 / PTK9

twinfilin actin-binding protein 1

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Gene Name: twinfilin actin-binding protein 1
Family/Subfamily: Protein Kinase , A6
Synonyms: TWF1, A6, A6 protein tyrosine kinase, Protein A6, Twinfilin, Twinfilin-1, Protein tyrosine kinase, Protein tyrosine kinase 9, PTK9, PTK9 protein tyrosine kinase 9
Target Sequences: NM_002822 NP_002813.3 Q12792

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Products
Antibodies (41)
Primary (41)
TWF1 / PTK9 (41)
Human (40)
Mouse (8)
Rat (8)
Bat (3)
Chimpanzee (3)
Dog (3)
Gibbon (6)
Guinea pig (3)
Horse (3)
Monkey (6)
Orangutan (3)
Pig (11)
Rabbit (3)
Xenopus (3)
Zebrafish (3)
IHC (6)
IHC-P (2)
WB (37)
Flo (2)
ELISA (7)
ICC (2)
IF (4)
Peptide-ELISA (2)
rabbit (26)
mouse (15)
IgG (8)
IgG1 (6)
IgG2a (2)
IgG2a,k (1)
IgG2b (6)
monoclonal mc (15)
polyclonal pc (26)
1E2 (1)
7C11 (2)
OTI10E6 (2)
OTI1C2 (2)
OTI4C3 (2)
OTI5C11 (2)
OTI5C12 (2)
OTI6C11 (2)
APC (1)
APC Conjugated (1)
Biotin Conjugated (4)
Carrier-free (6)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (25)
aa140-384 (12)
aa1-252 (8)
N-Terminus (4)
aa191-240 (4)
Amino Acid: 335-384 (1)
aa1-50 (1)
aa137-171 (1)
aa201-235 (1)
No (41)
TWF1 / PTK9 Antibody - IHC of paraffin-embedded ovarian cancer tissues using TWF1 mouse monoclonal antibody with DAB staining.
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TWF1 / PTK9 Mouse anti-Human Monoclonal (7C11) Antibody
Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$459
TWF1 / PTK9 Antibody - Sample: recombinant human TWF1.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal (aa1-252) Antibody
Pig, Human
WB
Unconjugated
100 µl/$319; 200 µl/$365; 500 µl/$519; 1 ml/$597; 2 ml/$826; 5 ml/$1,352
TWF1 / PTK9 Antibody - PTK9 Antibody in Immunohistochemistry (IHC)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (Amino Acid: 335-384) (7C11) Antibody
Human
Flo, ICC, IF, IHC, WB
Unconjugated
100 µg/$503
TWF1 / PTK9 Antibody - PTK9 monoclonal antibody (M02), clone 1E2 Western blot of PTK9 expression in HeLa.
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TWF1 / PTK9 Mouse anti-Human Monoclonal (1E2) Antibody
Rat, Human
ELISA, WB
Unconjugated
100 µg/$423
TWF1 / PTK9 Antibody - Immunohistochemistry of paraffin-embedded human pancreatic tissue at dilution of 1:100
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TWF1 / PTK9 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
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TWF1 / PTK9 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
TWF1 / PTK9 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (OTI5C12) Antibody
Human
IHC, WB
Unconjugated
100 µl/$434
TWF1 / PTK9 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TWF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (Carrier-free) (OTI5C12) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
TWF1 / PTK9 Antibody - Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PTK9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, WB
Unconjugated
100 µl/$450
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TWF1 / PTK9 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (OTI6C11) Antibody
Human
WB
Unconjugated
100 µl/$434
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1. (1:2000)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (Carrier-free) (OTI6C11) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (OTI10E6) Antibody
Human
WB
Unconjugated
100 µl/$434
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1. (1:2000)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (Carrier-free) (OTI10E6) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (OTI4C3) Antibody
Human
WB
Unconjugated
100 µl/$434
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1. (1:2000)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (Carrier-free) (OTI4C3) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (OTI5C11) Antibody
Human
WB
Unconjugated
100 µl/$434
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1. (1:2000)
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (Carrier-free) (OTI5C11) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed
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TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (OTI1C2) Antibody
Human
WB
Unconjugated
100 µl/$434
TWF1 / PTK9 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY TWF1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1. (1:2000)
Select
TWF1 / PTK9 Mouse anti-Human Monoclonal (aa140-384) (Carrier-free) (OTI1C2) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
TWF1 / PTK9 Antibody - All lanes: Anti-TWF1 Antibody (Center) at 1:2000 dilution. Lane 1: NCI-H1299 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal (aa201-235) Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
200 µl/$393
TWF1 / PTK9 Antibody - All lanes: Anti-TWF1 Antibody (Center) at 1:2000 dilution. Lane 1: NCI-H1299 whole cell lysate. Lane 2: HeLa whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal (aa137-171) Antibody
Human
Peptide-ELISA, WB
Unconjugated
200 µl/$393
TWF1 / PTK9 Antibody - TWF1 / PTK9 antibody Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
TWF1 / PTK9 Antibody - Detection of human TWF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TWF1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Human
WB
Unconjugated
10 µl/$295; 100 µl/$514
TWF1 / PTK9 Antibody - Western blot analysis of extracts of various cell lines, using TWF1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
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TWF1 / PTK9 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).