TOP1 / Topoisomerase I

topoisomerase (DNA) I

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

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Products

ELISA Kits (9)

Type

Custom (1)

Sandwich (8)

Target

TOP1 / Topoisomerase I (9)

Reactivity

Human (3)

Mouse (4)

Rat (2)

Format

96-Well Strip Plate (9)

Publications

No (9)

Sample Type

Cell Lysates (3)

Plasma (4)

Serum (4)

Tissue Homogenates (5)

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Human TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F12829

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.78 - 50 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$669

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Human TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F6459

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$730

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Mouse TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F12828

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$669

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Mouse TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F9083

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$748

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Rat TOP1 / Topoisomerase I (Custom ELISA) ELISA Kit - LS-F20486

Type:

Custom

Format:

96-Well Strip Plate

Reactivity:

Rat

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$669

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Rat TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F39007

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rat

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$783

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Mouse TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F33859

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

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Human TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F33858

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

1.56 - 100 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

Select

Mouse TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F48655

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

78.125 - 5000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$586

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