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SMEK1

SMEK homolog 1, suppressor of mek1 (Dictyostelium)

Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.

Gene Name: SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Synonyms: SMEK1, FLFL1, KIAA2010, MSTP033, SMEK homolog 1, Smk1, PP4R3A, PPP4R3A, Smk-1
Target Sequences: NM_032560 AAG39284.1

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Products
Antibodies (12)
Primary (12)
SMEK1 (12)
Human (12)
Mouse (7)
Rat (5)
Bat (4)
Bovine (4)
Chicken (4)
Chimpanzee (3)
Dog (1)
Gibbon (3)
Guinea pig (3)
Hamster (1)
Horse (4)
Monkey (4)
Pig (4)
Rabbit (4)
Zebrafish (4)
IHC (5)
IHC-P (1)
WB (9)
ICC (1)
IF (3)
IP (2)
Peptide-ELISA (1)
rabbit (11)
goat (1)
IgG (6)
polyclonal pc (12)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (9)
aa611-660 (4)
aa770-C-term (2)
aa600-650 (1)
aa645-657 (1)
No (12)
SMEK1 Antibody - Detection of Human PPP4R3 Alpha by Immunohistochemistry and Immunofluorescence. Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-PPP4R3 Alpha used at a dilution of 1:200 (1 ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.
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SMEK1 Rabbit anti-Human Polyclonal (aa770-C-term) Antibody
Human
IF, IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
SMEK1 Antibody - Detection of Human PPP4R3 Alpha by Immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PPP4R3 Alpha used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
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SMEK1 Rabbit anti-Human Polyclonal (aa770-C-term) Antibody
Mouse, Human
IF, IHC
Unconjugated
2.5 µg/$295; 100 µl/$532
SMEK1 Antibody - Immunohistochemistry of paraffin-embedded human stomach tissue.
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SMEK1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
SMEK1 Antibody - Immunofluorescence staining of SMEK1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SMEK1 polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
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SMEK1 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF
Unconjugated
100 µl/$412
SMEK1 Antibody
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SMEK1 Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321
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SMEK1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
SMEK1 Antibody - Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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SMEK1 Goat anti-Human Polyclonal (aa645-657) Antibody
Chicken, Zebrafish, Pig, Horse, Bat, Bovine, Rabbit, Mouse, Dog, Hamster, Human, Monkey
Peptide-ELISA, WB
Unconjugated
100 µg/$503
SMEK1 Antibody - SMEK1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMEK1 Rabbit anti-Human Polyclonal (aa611-660) Antibody
Human
WB
Unconjugated
100 µl/$424
SMEK1 Antibody - SMEK1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMEK1 Rabbit anti-Human Polyclonal (aa611-660) (HRP) Antibody
Chicken, Zebrafish, Rabbit, Mouse, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Gibbon, Human, Monkey, Chimpanzee
WB
HRP Conjugated
100 µl/$469
SMEK1 Antibody - SMEK1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMEK1 Rabbit anti-Human Polyclonal (aa611-660) (Biotin) Antibody
Chicken, Zebrafish, Rabbit, Mouse, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Gibbon, Human, Monkey, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
SMEK1 Antibody - SMEK1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMEK1 Rabbit anti-Human Polyclonal (aa611-660) (FITC) Antibody
Chicken, Zebrafish, Rabbit, Mouse, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Gibbon, Human, Monkey, Chimpanzee
WB
FITC Conjugated
100 µl/$469
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SMEK1 Rabbit anti-Human Polyclonal (aa600-650) Antibody
Human
IP
Unconjugated
10 µg/$295; 100 µl/$514
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).