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SERPINH1 / HSP47

serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)

SERPINH1 / HSP47 is a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.

Gene Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms: SERPINH1, 47 kDa heat shock protein, AsTP3, Colligin, Colligin-1, Collagen-binding protein, Colligin-2, CBP1, HSP47, OI10, Serpin H1, SERPINH2, CBP2, Collagen-binding protein 1, Colligen, gp46, PIG14, PPROM, RA-A47
Target Sequences: NM_001235 NP_001226.2 P50454

Publications (3)

1
Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures. Wong K, Sun F, Trudel G, Sebastiani P, Laneuville O. BMC musculoskeletal disorders. 2015 16:125. (ICC; Human, Rat) [Full Text Article] [PubMed:26006773] [PMC:PMC4443538]
2
Functional genomics study of acute heat stress response in the small yellow follicles of layer-type chickens. Cheng CY, Tu WL, Chen CJ, Chan HL, Chen CF, , Chen HH, Tang PC, Lee YP, Chen SE, , Huang SY, ,. Scientific reports. 2018 8:1320. (WB; Human) [Full Text Article] [PubMed:29358656]
3
Proteomic Analysis of Thermal Regulation of Small Yellow Follicles in Broiler-Type Taiwan Country Chickens. Chuen-Yu Cheng, Wei-Lin Tu, Chao-Jung Chen, Hong-Lin Chan, Chih-Feng Chen, Hsin-Hsin Chen, Pin-Chi Tang, Yen-Pai Lee, Shuen-Ei Chen, San-Yuan Huang. The journal of poultry science. 2018 January;55:120-136. [Full Text Article] [PubMed:32055165] [PMC:PMC6756493]

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Proteins (9)
Over-Expression Lysate (2)
Recombinant (6)
SERPINH1 / HSP47 (9)
No (9)
6His, C-terminus (2)
GST (1)
His (1)
His-SUMO (1)
Myc-DDK (Flag) (3)
Human (6)
Mouse (2)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Human Cells (1)
Mammalian Cells (1)
Wheat Germ Extract (1)
Yeast (1)
Purified (2)
Low endotoxin level (2)
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E. coli
His-SUMO
60.77 kD
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
45.5kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
SERPINH1 / HSP47 Protein
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Yeast
His
46.79 kD
100 µg/$499; 20 µg/$364; 1 mg/$1,683
SERPINH1 / HSP47 Protein - 12.5% SDS-PAGE of human SERPINH1 stained with Coomassie Blue
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Wheat Germ Extract
GST
71.72 kDa
10 µg/$479; 25 µg/$670
SERPINH1 / HSP47 Protein - Purified recombinant protein SERPINH1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
44.5 kDa
20 µg/$1,107
SERPINH1 / HSP47 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
46.47 kDa
20 µg/$150
SERPINH1 / HSP47 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
46.47 kDa
100 µg/$494
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E. coli
None
48.9 kDa
10 µg/$329; 50 µg/$412; 1000 µg/$2,312
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Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
45.5 kDa
10 µg/$317; 50 µg/$524
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).