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RFC5

replication factor C (activator 1) 5, 36.5kDa

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.

Gene Name: replication factor C (activator 1) 5, 36.5kDa
Synonyms: RFC5, Activator 1 subunit 5, A1 36 kDa subunit, Activator 1 36 kDa subunit, RF-C 36 kDa subunit, RFC, 36.5 kD subunit, RFC36, Replication factor C subunit 5
Target Sequences: NM_007370 NP_031396.1 P40937

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Proteins (5)
Over-Expression Lysate (2)
Recombinant (3)
RFC5 (5)
No (5)
GST (1)
His, N-Terminal (2)
Myc-DDK (Flag) (2)
Human (3)
Mouse (1)
293T Cells (1)
E. coli (2)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (1)
RFC5 Protein - 12.5% SDS-PAGE of human RFC5 stained with Coomassie Blue
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Wheat Germ Extract
GST
63.14 kDa
10 µg/$479; 25 µg/$670
RFC5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
38.3 kDa
20 µg/$150
RFC5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
38.3 kDa
100 µg/$494
RFC5 Protein - Recombinant Replication Factor C5 (RFC5) by SDS-PAGE
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E. coli
His, N-Terminal
28.5kDa
10 µg/$267; 50 µg/$352; 200 µg/$702; 100 µg/$562; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
RFC5 Protein - Recombinant Replication Factor C5 (RFC5) by SDS-PAGE
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E. coli
His, N-Terminal
25.2kDa
10 µg/$271; 50 µg/$382; 200 µg/$762; 100 µg/$610; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).