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RAD17

RAD17 homolog (S. pombe)

RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Gene Name: RAD17 homolog (S. pombe)
Synonyms: RAD17, HRAD17, R24L, RAD1 homolog, RAD1 (S. pombe) homolog, RAD24, Rad17-like protein, RF-C activator 1 homolog, CCYC, RAD17 homolog (S. pombe), RAD17SP, RF-C/activator 1 homolog
Target Sequences: NM_002873 NP_002864.1 O75943

Publications (2)

1
hRAD17, a structural homolog of the Schizosaccharomyces pombe RAD17 cell cycle checkpoint gene, stimulates p53 accumulation. Li L, Peterson CA, Kanter-Smoler G, Wei YF, Ramagli LS, Sunnerhagen P, Siciliano MJ, Legerski RJ. Oncogene. 1999 18:1689-99. (WB, Flo; Human) [PubMed:10208430]
2
ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses. Bao S, Tibbetts RS, Brumbaugh KM, Fang Y, Richardson DA, Ali A, Chen SM, Abraham RT, Wang XF. Nature. 2001 411:969-74. (Flo; Human) [PubMed:11418864]

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Products
Antibodies (46)
Primary (46)
RAD17 (46)
Human (45)
Mouse (21)
Rat (9)
Bat (3)
Bovine (3)
Dog (6)
Gibbon (3)
Hamster (3)
Monkey (4)
Orangutan (6)
Pig (6)
IHC (14)
IHC-P (7)
WB (41)
Flo (7)
ELISA (19)
ICC (2)
IF (9)
IP (4)
Peptide-ELISA (3)
rabbit (42)
mouse (2)
goat (2)
IgG (33)
IgG1 (1)
IgG1,k (1)
monoclonal mc (2)
polyclonal pc (44)
2G12 (1)
31E9 (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (32)
aa218-246 (7)
C-Terminus (5)
N-Terminus (4)
aa1-270 (4)
pSer645 (3)
590-670 aa (2)
580-660 aa (1)
Ala639 (1)
Ser635 (1)
aa1-200 (1)
aa2-15 (1)
aa564-614 (1)
aa621-670 (1)
p645 (1)
No (46)
RAD17 Antibody - IHC of Rad17 (A639) pAb in paraffin-embedded human breast carcinoma tissue.
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RAD17 Rabbit anti-Human Polyclonal (Ala639) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
RAD17 Antibody - Immunohistochemistry of paraffin-embedded human colon carcinoma tissue.
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RAD17 Rabbit anti-Human Polyclonal Antibody
Human
IF, IHC, IP, WB
Unconjugated
20 µl/$275; 100 µl/$389
RAD17 Antibody - Immunohistochemical analysis of RAD17 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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RAD17 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$299
RAD17 Antibody - Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rad17 (Ab-645) antibody
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RAD17 Rabbit anti-Human Polyclonal (p645) Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
RAD17 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAD17 Antibody. The picture on the right is blocked with the synthesized peptide.
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RAD17 Rabbit anti-Human Polyclonal (aa621-670) Antibody
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
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RAD17 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$331
RAD17 Antibody - Western blot of Rad17 antibody
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RAD17 Rabbit anti-Human Polyclonal (590-670 aa) Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
RAD17 Antibody
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RAD17 Mouse anti-Human Monoclonal (31E9) Antibody
Mouse, Rat, Human
IHC, IHC-P, IP, WB
Unconjugated
100 µg/$403
RAD17 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAD17 Rabbit anti-Human Polyclonal (aa1-270) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
RAD17 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAD17 Rabbit anti-Human Polyclonal (aa1-270) (HRP) Antibody
Human
ELISA, IHC, IHC-P
HRP Conjugated
50 µg/$294; 100 µg/$360
RAD17 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAD17 Rabbit anti-Human Polyclonal (aa1-270) (Biotin) Antibody
Human
ELISA, IHC, IHC-P
Biotin Conjugated
50 µg/$294; 100 µg/$360
RAD17 Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RAD17 Rabbit anti-Human Polyclonal (aa1-270) (FITC) Antibody
Human
ELISA, IHC, IHC-P
FITC Conjugated
50 µg/$294; 100 µg/$360
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RAD17 Rabbit anti-Human Polyclonal (aa218-246) Antibody
Human
ELISA, Flo, WB
Unconjugated
200 µl/$734
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RAD17 Rabbit anti-Human Polyclonal (aa218-246) (Biotin) Antibody
Human
ELISA, Flo, WB
Biotin Conjugated
200 µl/$952
Select
RAD17 Rabbit anti-Human Polyclonal (aa218-246) (FITC) Antibody
Human
ELISA, Flo, WB
FITC Conjugated
200 µl/$952
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RAD17 Rabbit anti-Human Polyclonal (aa218-246) (Azide-free) (HRP) Antibody
Human
ELISA, Flo, WB
HRP Conjugated, Azide-free
200 µl/$952
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RAD17 Rabbit anti-Human Polyclonal (aa218-246) (APC) Antibody
Human
ELISA, Flo, WB
APC Conjugated
200 µl/$952
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RAD17 Rabbit anti-Human Polyclonal (aa218-246) (PE) Antibody
Human
ELISA, Flo, WB
PE Conjugated
200 µl/$952
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RAD17 Rabbit anti-Human Polyclonal Antibody
Human
IF, IP, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
RAD17 Antibody - Immunofluorescence staining of RAD17 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RAD17 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
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RAD17 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF
Unconjugated
100 µl/$412
RAD17 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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RAD17 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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RAD17 Rabbit anti-Human Polyclonal (pSer645) Antibody
Mouse, Human
IHC, WB
Unconjugated
10 µl/$295; 100 µl/$533
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RAD17 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
RAD17 Antibody - RAD17 Antibody western blot of A549 cell line lysates (35 ug/lane). The RAD17 antibody detected the RAD17 protein (arrow).
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RAD17 Rabbit anti-Human Polyclonal (aa218-246) Antibody
Human
Flo, WB
Unconjugated
400 µl/$393
RAD17 Antibody - RAD17 monoclonal antibody (M01), clone 2G12 Western Blot analysis of RAD17 expression in HeLa NE.
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RAD17 Mouse anti-Human Monoclonal (2G12) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).