PKG / PRKG1

protein kinase, cGMP-dependent, type I

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca2+ release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca2+ levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

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Products

ELISA Kits (10)

Type

Sandwich (9)

Sandwich CLIA (1)

Target

PKG / PRKG1 (10)

Reactivity

Human (4)

Mouse (1)

Chicken (1)

Guinea pig (1)

Monkey (1)

Porcine (1)

Rabbit (1)

Format

12 x 8-Well Microstrips (1)

96-Well Microplate (1)

96-Well Strip Plate (8)

Publications

No (10)

Sample Type

Cell Lysates (1)

Plasma (3)

Serum (3)

Tissue Homogenates (3)

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Mouse PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F9645

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$748

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Human PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F21272

Type:

Sandwich

Format:

96-Well Microplate

Reactivity:

Human

Range:

0.16 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$586

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Human PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F7540

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Tissue Homogenates

Price:

1 Plate/$730

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Human PKG / PRKG1 (CLIA) ELISA Kit - LS-F30383

Type:

Sandwich CLIA

Format:

12 x 8-Well Microstrips

Reactivity:

Human

Range:

31.25 - 2000 pg/ml

Detection:

Chemiluminescent

Sample Types:

Plasma, Serum

Price:

1 Plate/$656

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Guinea pig PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F47427

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Guinea pig

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$656

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Chicken PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F47428

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Chicken

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$656

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Monkey PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F47429

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Monkey

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$656

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Rabbit PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F47430

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rabbit

Range:

62.5 - 4000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$656

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Porcine PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F47431

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Porcine

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$656

Select

Human PKG / PRKG1 (Sandwich ELISA) ELISA Kit - LS-F32809

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum, Tissue Homogenates

Price:

1 Plate/$579

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