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PDIA3 / ERp57

protein disulfide isomerase family A, member 3

PDIA3 / ERp57 encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Gene Name: protein disulfide isomerase family A, member 3
Synonyms: PDIA3, 58 kDa microsomal protein, ER protein 57, ERp60, ERp61, Disulfide isomerase ER-60, Endoplasmic reticulum P58, GRP58, HsT17083, p58, Phospholipase C-alpha, PI-PLC, ER protein 60, ER60, ERp57, GRP57, Protein disulfide-isomerase A3
Target Sequences: NM_005313 NP_005304.3 P30101

Publications (2)

1
Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Chamberlain N, Korwin-Mihavics BR, Nakada EM, Bruno SR, Heppner DE, Chapman DG, Hoffman SM, van der Vliet A, Suratt BT, Dienz O, Alcorn JF, Anathy V. Redox biology. 2019 April;22:101129. [Full Text Article] [PubMed:30735910] [PMC:PMC6365984] Related Antibodies: LS-B9768.
2
Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung. Nicolas Chamberlain, Mona Ruban, Zoe F Mark, Sierra R Bruno, Amit Kumar, Ravishankar Chandrasekaran, Dhemerson Souza De Lima, Danielle Antos, Emily M Nakada, John F Alcorn, Vikas Anathy. International journal of molecular sciences. 2022 January;23:1078. [Full Text Article] [PubMed:35162999] [PMC:PMC8834910] Related Antibodies: LS-B9768.

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Proteins (10)
Over-Expression Lysate (2)
Recombinant (8)
PDIA3 / ERp57 (10)
No (10)
10His, N-terminus + Myc, C-terminus (1)
6His, C-terminus (2)
GST (1)
GST, N-terminus (1)
His (2)
Myc-DDK (Flag) (3)
Human (9)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Human Cells (1)
Mammalian Cells (1)
Wheat Germ Extract (2)
Purified (2)
Low endotoxin level (2)
PDIA3 / ERp57 Protein
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E. coli
His
100 µg/$342; 500 µg/$641; 20 µg/$254
PDIA3 / ERp57 Protein - Purified recombinant protein PDIA3 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
54.2 kDa
20 µg/$1,107
PDIA3 / ERp57 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His, N-terminus + Myc, C-terminus
59.3 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
55.3 kDa
10 µg/$344; 50 µg/$584
PDIA3 / ERp57 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
56.78 kDa
100 µg/$494
PDIA3 / ERp57 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
56.78 kDa
20 µg/$150
PDIA3 / ERp57 Protein - Recombinant Protein Disulfide Isomerase A3 By SDS-PAGE
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E. coli
His
30.8 kDa
10 µg/$271; 50 µg/$382; 100 µg/$610; 200 µg/$762; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
PDIA3 / ERp57 Protein - 12.5% SDS-PAGE of human PDIA3 stained with Coomassie Blue
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Wheat Germ Extract
GST
83.2 kDa
10 µg/$479; 25 µg/$670
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
55.3kD
10 µg/$321; 50 µg/$534; 500 µg/$1,680; 1 mg/$2,353
PDIA3 / ERp57 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).