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Oxytocin

oxytocin/neurophysin I prepropeptide

Oxytocin is a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

Gene Name: oxytocin/neurophysin I prepropeptide
Family/Subfamily: Ligand , not assigned-Ligand
Synonyms: OXT, OXT-NPI, Oxytocin, OT, Oxytocin-neurophysin 1, Neurophysin I, OT-NPI
Target Sequences: NM_000915 NP_000906.1 P01178

Publications (2)

1
The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention. Anna Rapacz-Leonard, Mark Leonard, Malgorzata Chmielewska-Krzesinska, Marta Siemieniuch & Tomasz E. Janowski. Scientific reports. 2020 February;10: (WB, IHC-P; Horse) [Full Text Article] [PubMed:32034259] [PMC:PMC7005845] Related Antibodies: LS-C145973.
2
Oxytocin modulates proliferation and stress responses of human skin cells: implications for atopic dermatitis. Deing V, Roggenkamp D, K[Character fc]hnl J, Gruschka A, St[Character e4]b F, Wenck H, B[Character fc]rkle A, Neufang G. Experimental dermatology. 2013 Jun;22:399-405. [Full Text Article] [PubMed:23711064] Related Antibodies: LS-C37953.

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Proteins (13)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (3)
Recombinant (9)
Oxytocin (13)
No (13)
10His, N-terminus + Myc, C-terminus (1)
10His-SUMO, N-terminus + Myc, C-terminus (1)
6His, N-terminus (1)
6His-SUMOstar, N-terminus (1)
His, N-Terminal (2)
His-T7, N-terminus (3)
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Human (10)
Mouse (1)
Rat (1)
293T Cells (1)
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HEK 293 Cells (4)
Yeast (3)
Purified (5)
Oxytocin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$570; 20 µg/$395; 1 mg/$1,973
Oxytocin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His, N-terminus + Myc, C-terminus
1 mg/$1,486; 100 µg/$451; 20 µg/$339
Oxytocin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
None
10.9 kDa
100 µg/$707; 20 µg/$478; 1 mg/$2,084
Oxytocin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His-SUMOstar, N-terminus
26.9 kDa
100 µg/$570; 20 µg/$395; 1 mg/$1,973
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HEK 293 Cells
His, N-Terminal
~10 kDa
200 µl/$283
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HEK 293 Cells
His, N-Terminal
~10 kDa
100 µg/$298
Oxytocin Protein - Recombinant Preprooxytocin (OT) by SDS-PAGE
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E. coli
His-T7, N-terminus
14.6kDa
10 µg/$283; 50 µg/$452; 200 µg/$902; 100 µg/$722; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204
Oxytocin Protein - Recombinant Preprooxytocin (OT) by SDS-PAGE
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E. coli
His-T7, N-terminus
14.6kDa
10 µg/$287; 50 µg/$482; 200 µg/$962; 100 µg/$770; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
Oxytocin Protein - Recombinant Preprooxytocin (OT) by SDS-PAGE
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E. coli
His-T7, N-terminus
14.5kDa
10 µg/$289; 50 µg/$492; 200 µg/$982; 100 µg/$785; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
Oxytocin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
12.72 kDa
20 µg/$150
Oxytocin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-SUMO, N-terminus + Myc, C-terminus
1 mg/$1,486; 100 µg/$451; 20 µg/$339
Oxytocin Protein - Purified recombinant protein OXT was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
10.9 kDa
20 µg/$1,107
Oxytocin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
12.72 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).