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NEFM / NF-M

neurofilament, medium polypeptide

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene Name: neurofilament, medium polypeptide
Synonyms: NEFM, 160 kDa neurofilament protein, NEF3, NF-M, NFM, Neurofilament 3
Target Sequences: NM_005382 NP_005373.2 P07197

Publications (3)

1
Enhancement of the growth and differentiation of nasal olfactory mucosa cells by the conjugation of growth factors to functional nanoparticles. Skaat H, Ziv-Polat O, Shahar A, Margel S. Bioconjugate chemistry. 2011 22:2600-10. (ICC; Rat) [PubMed:22029397]
2
Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering. Ziv-Polat O, Skaat H, Shahar A, Margel S. International journal of nanomedicine. 2012 7:1259-74. (ICC; Rat) [PubMed:22419873] [PMC:PMC3298389]
3
Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Louis ED, Ma K, Babij R, Corts E, Liem RK, Vonsattel JP, Faust PL. Neuroscience letters. 2012 518:49-54. (IHC, WB; Human) [PubMed:22561033] [PMC:PMC3364538]

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Proteins (5)
Over-Expression Lysate (2)
Recombinant (3)
NEFM / NF-M (5)
No (5)
10His, N-terminus + Myc, C-terminus (1)
His, N-Terminal (1)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Purified (1)
NEFM / NF-M Protein - Recombinant Neurofilament 3 By SDS-PAGE
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E. coli
His, N-Terminal
26.2 kDa
10 µg/$285; 50 µg/$467; 100 µg/$747; 200 µg/$932; 1 mg/$2,058; 500 µg/$1,590; 5 mg/$3,263; 2 mg/$2,246
NEFM / NF-M Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
102.3 kDa
20 µg/$215
NEFM / NF-M Protein - Purified recombinant protein NEFM was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
102.3 kDa
20 µg/$1,107
NEFM / NF-M Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
102.3 kDa
100 µg/$710
NEFM / NF-M Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His, N-terminus + Myc, C-terminus
34.8 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).