NCBP1 / CBP80

nuclear cap binding protein subunit 1, 80kDa

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

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Proteins (6)

Type

Over-Expression Lysate (2)

Recombinant (4)

Target

NCBP1 / CBP80 (6)

Publications

No (6)

Tag

GST (2)

GST, N-terminus (1)

Myc-DDK (Flag) (3)

Species

Human (5)

Source

293T Cells (1)

HEK 293 Cells (2)

Wheat Germ Extract (3)

Purification

Purified (2)

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Human NCBP1 / CBP80 Protein (Recombinant GST) (Full Length) - LS-G29447

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

118.2 kDa

Price:

10 µg/$479; 25 µg/$670

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Human NCBP1 / CBP80 Protein (Recombinant GST, N-terminus) (aa2-99) - LS-G59351

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human NCBP1 / CBP80 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G64813

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

91.7 kDa

Price:

20 µg/$1,107

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Human NCBP1 / CBP80 Protein (Recombinant GST) (Full Length) - LS-G29445

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

112.64 kDa

Price:

10 µg/$479; 25 µg/$670

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Human NCBP1 / CBP80 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G74265

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

91.7 kDa

Price:

20 µg/$150

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NCBP1 / CBP80 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G117956

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

91.7 kDa

Price:

100 µg/$494

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