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MMP13

matrix metallopeptidase 13 (collagenase 3)

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

Gene Name: matrix metallopeptidase 13 (collagenase 3)
Family/Subfamily: Protease , Metallopeptidase M10A
Synonyms: MMP13, Collagenase 3, Matrix metalloproteinase-13, Interstitial collagenase, MANDP1, MMP-13, CLG3
Target Sequences: NM_002427 NP_002418.1 P45452

Publications (6)

1
Comparison of gait and pathology outcomes of three meniscal procedures for induction of knee osteoarthritis in sheep. Cake MA, Read RA, Corfield G, Daniel A, Burkhardt D, Smith MM, Little CB. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society. 2013 21:226-36. (IHC; Sheep) [PubMed:23069853]
2
The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs. Sun Y, Kiraly AJ, Cox M, Mauerhan DR, Hanley EN Jr. Experimental and therapeutic medicine. 2018 April;15:3320-3328. (Rat) [Full Text Article] [PubMed:29545850] [PMC:PMC5841021]
3
Short-Term Intake of a Fructose-, Fat- and Cholesterol-Rich Diet Causes Hepatic Steatosis in Mice: Effect of Antibiotic Treatment. Brandt A, Jin CJ, Nolte K, Sellmann C, Engstler AJ, Bergheim I. Nutrients. 2017 September;9:pii: E1013. (Rat) [Full Text Article] [PubMed:28906444] [PMC:PMC5622773]
4
Targeted designed variants of alpha-2-macroglobulin (A2M) attenuate cartilage degeneration in a rat model of osteoarthritis induced by anterior cruciate ligament transection. Zhang Y, Wei X, Browning S, Scuderi G, Hanna LS, Wei L. Arthritis research & therapy. 2017 July;19:175. [Full Text Article] [PubMed:28743292] [PMC:PMC5526282]
5
Aspirin-triggered lipoxin A4 inhibits atherosclerosis progression in apolipoprotein E-/-<sup> mice. Petri MH, Laguna-Fernandez A, Arnardottir H, Wheelock CE, Perretti M, Hansson GK, Bäck M. British journal of pharmacology. 2017 November;174:4043-4054. [Full Text Article] [PubMed:28071789] [PMC:PMC5659998]
6
Atelocollagen promotes chondrogenic differentiation of human adipose-derived mesenchymal stem cells. Seon Ae Kim, Yoo Joon Sur, Mi-La Cho, Eun Jeong Go, Yun Hwan Kim, Asode Ananthram Shetty, Seok Jung Kim. Scientific reports. 2020 June;10:10678. [Full Text Article] [PubMed:32606308] [PMC:PMC7327030]
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Proteins (17)
Over-Expression Lysate (2)
Recombinant (15)
MMP13 (17)
No (17)
6His-SUMO, N-terminus (1)
GST, N-terminus (1)
His (7)
His, N-Terminal (3)
His, N-terminus (1)
His-SUMO (1)
His-T7 (1)
Myc-DDK (Flag) (2)
Dog (1)
Human (6)
Mouse (2)
Pig (1)
Rabbit (2)
Rat (4)
293F Cells (2)
293T Cells (1)
E. coli (12)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (4)
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His, N-Terminal
22.2 kDa
10 µg/$286; 50 µg/$472; 200 µg/$942; 1 mg/$2,069; 5 mg/$3,291; 2 mg/$2,257
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His, N-terminus
19.1 kDa
10 µg/$288; 50 µg/$487; 100 µg/$780; 200 µg/$972; 1 mg/$2,109; 500 µg/$1,636; 5 mg/$3,403; 2 mg/$2,298
MMP13 Protein
Select
E. coli
His
22.6 kDa
10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204
MMP13 Protein
Select
E. coli
His-SUMO
58.3 kD
100 µg/$420; 1 mg/$1,355; 20 µg/$323
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His
21.0 kDa
10 µg/$286; 50 µg/$472; 100 µg/$752; 200 µg/$942; 1 mg/$2,069; 500 µg/$1,600; 5 mg/$3,291; 2 mg/$2,257
MMP13 Protein - Recombinant  Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His-T7
20.1 kDa
10 µg/$294; 50 µg/$522; 100 µg/$832; 200 µg/$1,034; 1 mg/$2,192; 500 µg/$1,712; 5 mg/$3,641; 2 mg/$2,383
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His
20.9 kDa
10 µg/$293; 50 µg/$517; 100 µg/$827; 200 µg/$1,026; 1 mg/$2,182; 500 µg/$1,702; 5 mg/$3,613; 2 mg/$2,373
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His
20.3 kDa
10 µg/$293; 50 µg/$517; 100 µg/$827; 200 µg/$1,026; 1 mg/$2,182; 500 µg/$1,702; 5 mg/$3,613; 2 mg/$2,373
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His
22.6 kDa
10 µg/$279; 50 µg/$432; 100 µg/$690; 200 µg/$862; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148
MMP13 Protein - Recombinant Matrix Metalloproteinase 13 By SDS-PAGE
Select
E. coli
His
22.0 kDa
10 µg/$288; 50 µg/$487; 100 µg/$780; 200 µg/$972; 1 mg/$2,109; 500 µg/$1,636; 5 mg/$3,403; 2 mg/$2,298
MMP13 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
MMP13 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
MMP13 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
53.82 kDa
20 µg/$150
MMP13 Protein - Active Matrix Metalloproteinase 13 (MMP13) by WB
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293F Cells
His, N-Terminal
60kDa
10 µg/$382; 50 µg/$952; 200 µg/$1,612; 100 µg/$1,378; 1 mg/$3,041; 500 µg/$2,437; 5 mg/$6,651; 2 mg/$3,548
MMP13 Protein - Eukaryotic Matrix Metalloproteinase 13 (MMP13) by SDS-PAGE
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293F Cells
His, N-Terminal
60kDa
10 µg/$288; 50 µg/$487; 200 µg/$972; 100 µg/$780; 1 mg/$2,109; 500 µg/$1,636; 5 mg/$3,403; 2 mg/$2,298
MMP13 Protein
Select
E. coli
His
100 µg/$739; 50 µg/$523; 500 µg/$1,917; 10 µg/$292; 20 µg/$361; 250 µg/$1,285
MMP13 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
53.82 kDa
100 µg/$494
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).