MLST8 / GBL

MTOR associated protein, LST8 homolog (S. cerevisiae)

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

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Proteins (4)

Type

Over-Expression Lysate (2)

Recombinant (2)

Target

MLST8 / GBL (4)

Publications

No (4)

Tag

GST (1)

His-T7 (1)

Myc-DDK (Flag) (2)

Species

Human (3)

Source

293T Cells (1)

E. coli (1)

HEK 293 Cells (1)

Wheat Germ Extract (1)

Purification

Purified (1)

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Human MLST8 / GBL Protein (Recombinant GST) (Full Length) - LS-G34962

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

62.3 kDa

Price:

10 µg/$479; 25 µg/$670

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Human MLST8 / GBL Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G83232

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

35.7 kDa

Price:

20 µg/$150

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MLST8 / GBL Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G110379

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

35.7 kDa

Price:

100 µg/$494

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Human MLST8 / GBL Protein (Recombinant His-T7) (aa69-247) - LS-G16237

Source:

E. coli

Tag:

His-T7

Molecular Weight:

23.7 kDa

Price:

10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204

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