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MED1 / TRAP220

mediator complex subunit 1

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize.

Gene Name: mediator complex subunit 1
Synonyms: MED1, ARC205, CRSP1, DRIP205, DRIP230, Mediator complex subunit 1, PBP, PPARG binding protein, RB18A, TRIP-2, TRIP2, PPARBP, CRSP200, p53 regulatory protein RB18A, PPAR binding protein, PPAR-binding protein, PPARGBP, TR-interacting protein 2, TRAP220
Target Sequences: NM_004774 NP_004765.2 Q15648

Publications (1)

1
The onset of human ectopic pregnancy demonstrates a differential expression of miRNAs and their cognate targets in the Fallopian tube. Feng Y, Zou S, Weijdegård B, Chen J, Cong Q, Fernandez-Rodriguez J, Wang L, Billig H, Shao R. International journal of clinical and experimental pathology. 2014 7:64-79. (IHC; Human) [PubMed:24427327] [PMC:PMC3885461] Related Antibodies: LS-C119067.

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Products
Antibodies (36)
Primary (36)
MED1 / TRAP220 (36)
Human (35)
Mouse (21)
Rat (4)
Monkey (3)
IHC (19)
IHC-P (7)
WB (28)
ELISA (12)
ICC (5)
IF (14)
IP (4)
Peptide-ELISA (6)
rabbit (34)
mouse (1)
goat (1)
Product Group
IHCPlus (2)
IgG (22)
IgG1,k (1)
monoclonal mc (1)
polyclonal pc (34)
recombinant monoclonal rmc (1)
2A2 (1)
APC (1)
APC Conjugated (1)
Biotin Conjugated (2)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (26)
aa1-212 (8)
pThr1457 (5)
1400-1480 aa (2)
aa1525-C-term (2)
610-690 aa, Internal (1)
Ab-1457 (1)
Internal (1)
Pro697 (1)
aa1423-1472 (1)
aa1531-1581 (1)
aa641-690 (1)
No (35)
Yes (1)
MED1 / TRAP220 Antibody - Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified goat anti-CRSP1/TRAP220 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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MED1 / TRAP220 Goat anti-Human Polyclonal (aa1531-1581) Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
10 µg/$295; 100 µl/$514
MED1 / TRAP220 Antibody - Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
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MED1 / TRAP220 Mouse anti-Human Monoclonal (2A2) Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$335
MED1 / TRAP220 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED1 Antibody. The picture on the right is blocked with the synthesized peptide.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (aa641-690) Antibody
Mouse, Human
IHC, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
MED1 / TRAP220 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
MED1 / TRAP220 Antibody - Immunohistochemical analysis of TRAP220 (pT1457) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (pThr1457) Antibody
Mouse, Rat, Human, Monkey
ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
30 µl/$263; 50 µl/$279; 100 µl/$315; 200 µl/$379
MED1 / TRAP220 Antibody - Western blot of TRAP220 antibody
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (610-690 aa, Internal) Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
MED1 / TRAP220 Antibody - Immunofluorescence analysis of HeLa cells, using PPAR-BP Antibody. The picture on the right is blocked with the synthesized peptide.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (aa1423-1472) Antibody
Mouse, Human
IF, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
MED1 / TRAP220 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (pThr1457) Antibody
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$282; 200 µl/$350
MED1 / TRAP220 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (aa1525-C-term) Antibody
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50 µl/$460
MED1 / TRAP220 Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human heart tissue using PPAR-ß (Phospho-Thr1457) antibody.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (pThr1457) Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
MED1 / TRAP220 Antibody - Western blot of MED1 / TRAP220 antibody.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (aa1-212) Antibody
Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
MED1 / TRAP220 Antibody - 1:100 staining mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$282; 200 µl/$350
MED1 / TRAP220 Antibody - Western blot of PPAR-BP phosphorylation expression in Serum treated HUVEC whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (pThr1457) Antibody
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
50 µl/$305; 100 µl/$357
MED1 / TRAP220 Antibody - Immunohistochemistry analysis of paraffin-embedded human heart, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (pThr1457) Antibody
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (1400-1480 aa) Antibody
Mouse, Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
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MED1 / TRAP220 Rabbit Recombinant Monoclonal Antibody
ICC, IHC, IP, WB
Unconjugated
20 µl/$299; 100 µl/$425
MED1 / TRAP220 Antibody - Western blot of PPAR-BP expression in HUVEC whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ICC, IF, WB
Unconjugated
50 µl/$305; 100 µl/$357
MED1 / TRAP220 Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$282; 200 µl/$350
MED1 / TRAP220 Antibody - Western blot of TRAP220 antibody
Select
MED1 / TRAP220 Rabbit anti-Human Polyclonal (1400-1480 aa) Antibody
Mouse, Human
ELISA, IF, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
MED1 / TRAP220 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MED1 antibody.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
100 µg/$357
MED1 / TRAP220 Antibody - Immunohistochemistry of paraffin-embedded human placenta tissue at dilution of 1:100
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
MED1 / TRAP220 Antibody - IHC of TRAP220 (P697) pAb in paraffin-embedded human breast carcinoma tissue.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (Pro697) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
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MED1 / TRAP220 Rabbit anti-Human Polyclonal (Ab-1457) Antibody
Mouse, Human
ELISA, IF
Unconjugated
100 µg/$357
MED1 / TRAP220 Antibody - Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
MED1 / TRAP220 Antibody - Western blot analysis of extracts of Jurkat cells, using MED1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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MED1 / TRAP220 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).