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MANF / ARMET

mesencephalic astrocyte-derived neurotrophic factor

MANF / ARMET is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.

Gene Name: mesencephalic astrocyte-derived neurotrophic factor
Synonyms: MANF, ARP, ARMET, Arginine-rich protein, Protein ARMET
Target Sequences: NM_006010 NP_006001.4 P55145

Publications (5)

1
Piperine ameliorates SCA17 neuropathology by reducing ER stress. Guo J, Cui Y, Liu Q, Yang Y, Li Y, Weng L, Tang B, Jin P, Li XJ, Yang S, Li S. Molecular neurodegeneration. 2018 January;13:4. (Human) [Full Text Article] [PubMed:29378605] [PMC:PMC5789626] Related Antibodies: LS-B2688.
2
Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice. Galli E, Rossi J, Neumann T, Andressoo JO, Drinda S, Lindholm P. Science advances. 2019 October;9:14318. [Full Text Article] [PubMed:31586115] [PMC:PMC6778185] Related Antibodies: LS-B2688.
3
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Is Highly Expressed in Mouse Tissues With Metabolic Function. Danilova T, Galli E, Pakarinen E, Palm E, Lindholm P, Saarma M, Lindahl M. Frontiers in endocrinology. 2019 November;10: [Full Text Article] [PubMed:31781038] [PMC:PMC6851024] Related Antibodies: LS-B2688.
4
MANF Is Neuroprotective in Early Stages of EAE, and Elevated in Spinal White Matter by Treatment With Dexamethasone. Jinhan Nam, Tapani K Koppinen, Merja H Voutilainen. Frontiers in cellular neuroscience. 2021 Jul;15:640084. [Full Text Article] [PubMed:34305531] [PMC:PMC8292680] Related Antibodies: LS-B2688.
5
MANF protein expression is upregulated in immune cells in the ischemic human brain and systemic recombinant MANF delivery in rat ischemic stroke model demonstrates anti-inflammatory effects. Jenni E Anttila, Olli S Mattila, Hock-Kean Liew, Kert Mätlik, Eero Mervaala, Päivi Lindholm, Maria Lindahl, Perttu J Lindsberg, Kuan-Yin Tseng, Mikko Airavaara. Acta neuropathologica communications. 2024 January;12:10. [Full Text Article] [PubMed:38229173] [PMC:PMC10792833] Related Antibodies: LS-B2688.
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☰ Filters
Products
Proteins (15)
Over-Expression Lysate (2)
Recombinant (13)
MANF / ARMET (15)
No (15)
6His, C-terminus (2)
Fc (1)
GST (2)
His (2)
His, N-Terminal (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (13)
Mouse (1)
293F Cells (1)
293T Cells (1)
E. coli (5)
HEK 293 Cells (2)
Human Cells (3)
Mammalian Cells (1)
Wheat Germ Extract (1)
Purified (7)
Low endotoxin level (6)
Biologically active (3)
MANF / ARMET Protein - The ED50 was found to be <0.5ng/ml using dose dependent proliferation of human pancreatic cells.
Select
E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
~18.4 kDa
40 µg/$291; 200 µg/$613; 1 mg/$2,269
MANF / ARMET Protein
Select
E. coli
None
Biologically active
18.2 kDa
5 µg/$282; 100 µg/$957; 500 µg/$1,747
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
18.1 kDa
5 µg/$268; 25 µg/$349
MANF / ARMET Protein - 12.5% SDS-PAGE of human ARMET stained with Coomassie Blue
Select
Wheat Germ Extract
GST
45.65 kDa
10 µg/$479; 25 µg/$670
Select
Human Cells
Fc
Less than 1.0 EU/µg protein (determined by LAL method).
44.9 kDa
100 µg/$556
MANF / ARMET Protein - Purified recombinant protein MANF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
18.1 kDa
20 µg/$1,107
MANF / ARMET Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
21.6 kDa
20 µg/$150
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
19.2 kDa
10 µg/$281; 50 µg/$389
Select
Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
21.5kD
10 µg/$281; 50 µg/$395; 500 µg/$1,585; 1 mg/$2,157
Select
Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
19.6 kDa
100 µg/$556
MANF / ARMET Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
MANF / ARMET Protein
Select
GST
33.44kDa
10 µg/$479; 25 µg/$670
Select
293F Cells
His, N-Terminal
21kDa
10 µg/$278; 50 µg/$422; 200 µg/$842; 100 µg/$672; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
MANF / ARMET Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
21.6 kDa
100 µg/$494
MANF / ARMET Protein - Recombinant Mesencephalic Astrocyte Derived Neurotrophic Factor By SDS-PAGE
Select
E. coli
His-T7
21.6 kDa
10 µg/$276; 50 µg/$412; 100 µg/$657; 200 µg/$822; 1 mg/$1,905; 500 µg/$1,455; 5 mg/$2,871; 2 mg/$2,090
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).