ISG15

ISG15 ubiquitin-like modifier

Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.

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Products

Proteins (10)

Type

Recombinant (10)

Target

ISG15 (10)

Product Group

Coronavirus Antibodies Proteins cDNAs (10)

SARS-CoV-2 Associated Proteins (10)

Publications

No (10)

Tag

6His, C-terminus (1)

GST (1)

GST, N-terminus (1)

His, N-Terminal (1)

His,C-terminus (1)

His-T7 (1)

Myc-DDK (Flag) (1)

Species

Human (9)

Mouse (1)

Source

E. coli (7)

HEK 293 Cells (1)

Wheat Germ Extract (2)

Purification

Purified (3)

Low Endotoxin Level

Low endotoxin level (2)

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Mouse ISG15 Protein (Recombinant His, N-Terminal) (aa2-161) - LS-G26127

Source:

E. coli

Tag:

His, N-Terminal

Molecular Weight:

21.5 kDa

Price:

10 µg/$284; 50 µg/$460; 100 µg/$735; 200 µg/$917; 1 mg/$2,037; 5 mg/$3,207; 2 mg/$2,225

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Human ISG15 Protein (Recombinant) - LS-G3482

Source:

E. coli

Tag:

None

Price:

100 µg/$317; 500 µg/$526; 20 µg/$245

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Human ISG15 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G67261

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

17.7 kDa

Price:

20 µg/$1,107

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Human ISG15 Protein (Recombinant GST, N-terminus) (aa1-100) - LS-G61207

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human ISG15 Protein (Recombinant His-T7) (aa2-157) - LS-G26126

Source:

E. coli

Tag:

His-T7

Molecular Weight:

20.7 kDa

Price:

10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176

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Human ISG15 Protein (Recombinant GST) (Full Length) - LS-G31744

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

44.3 kDa

Price:

10 µg/$479; 25 µg/$670

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Human ISG15 Protein (Recombinant) (Met1-Ser165) - LS-G97624

Source:

E. coli

Tag:

None

Molecular Weight:

17.9 kDa

Price:

1 mg/$499

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Human ISG15 Protein (Recombinant His,C-terminus) (Gly2-Gly157) - LS-G138800

Source:

E. coli

Tag:

His,C-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

18.2 kDa

Price:

10 µg/$332; 50 µg/$376

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Human ISG15 Protein (Recombinant) (Met1-Gly157) - LS-G97623

Source:

E. coli

Tag:

None

Molecular Weight:

17.2 kDa

Price:

1 mg/$443

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Human ISG15 Protein (Recombinant 6His, C-terminus) (Gly2-Gly157) - LS-G39730

Source:

E. coli

Tag:

6His, C-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

18.2kD

Price:

10 µg/$243; 50 µg/$288; 500 µg/$1,262; 1 mg/$1,627

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