INSR / Insulin Receptor

insulin receptor

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

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Products

Proteins (19)

Type

Over-Expression Cell Culture Supernatant (1)

Over-Expression Lysate (6)

Recombinant (12)

Target

INSR / Insulin Receptor (19)

Application

Bind (1)

ELISA (1)

Func (1)

SDS-PAGE (1)

Publications

No (19)

Tag

6His, N-terminus (3)

6His-SUMO, N-terminus (1)

DDK (Flag)-His, C-terminus (1)

GST, N-terminus (1)

His (6)

His,C-terminus (1)

His-GST (1)

His-T7 (1)

Myc-DDK (Flag) (4)

Species

Human (15)

Mouse (1)

Rat (1)

Source

293T Cells (2)

Baculovirus (1)

E. coli (6)

HEK 293 Cells (7)

Human Cells (2)

Wheat Germ Extract (1)

Purification

Purified (2)

Low Endotoxin Level

Low endotoxin level (4)

Biologically Active

Biologically active (1)

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Human INSR / Insulin Receptor Protein (Over-Expression Lysate 6His, N-terminus) - LS-G26721

Source:

HEK 293 Cells

Tag:

6His, N-terminus

Molecular Weight:

112 kDa

Price:

100 µg/$298

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Human INSR / Insulin Receptor Protein (Recombinant His,C-terminus) (Met1-Lys944) - LS-G48193

Source:

HEK 293 Cells

Tag:

His,C-terminus

Application:

Bind ELISA Func SDS-PAGE

Biological Activity:

Biologically active

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

The secreted recombinant human INSR isoform short consists of 928 amino acids and has a predicted molecular mass of 106 (83+23) kDa. As a result of glycosylation, the apparent molecular mass of rhINSR is approximately 125-135 kDa & 40-45 kDa, corresponding to the a subunit and the ECD of ß subunit respectively in SDS-PAGE under reducing conditions.

Price:

100 µg/$441; 1 mg/$2,003

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Human INSR / Insulin Receptor Protein (Recombinant GST, N-terminus) (aa881-980) - LS-G59004

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human INSR / Insulin Receptor Protein (Over-Expression Lysate 6His, N-terminus) (Arg980-Ser1382) - LS-G26742

Source:

HEK 293 Cells

Tag:

6His, N-terminus

Molecular Weight:

46 kDa

Price:

100 µg/$298

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Human INSR / Insulin Receptor Protein (Recombinant His) (aa1027-1364) - LS-G13078

Source:

E. coli

Tag:

His

Molecular Weight:

39.6 kDa

Price:

10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900

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Mouse INSR / Insulin Receptor Protein (Recombinant His) (aa1101-1372) - LS-G13086

Source:

E. coli

Tag:

His

Molecular Weight:

32.6 kDa

Price:

10 µg/$282; 50 µg/$450; 100 µg/$717; 200 µg/$897; 1 mg/$2,010; 500 µg/$1,547; 5 mg/$3,137; 2 mg/$2,198

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Human INSR / Insulin Receptor Protein (Recombinant His-GST) (Gly989-Ser1382) - LS-G97621

Source:

Baculovirus

Tag:

His-GST

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

72.3 kDa

Price:

50 µg/$707

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Human INSR / Insulin Receptor Protein (Recombinant His) (Met1-Lys944) - LS-G98605

Source:

Human Cells

Tag:

His

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

106 kDa

Price:

100 µg/$721

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Human INSR / Insulin Receptor Protein (Recombinant His) (Met1-Lys956) - LS-G98606

Source:

Human Cells

Tag:

His

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

107 kDa

Price:

100 µg/$721; 1 mg/$4,701

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Human INSR / Insulin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G81376

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

156.33 kDa

Price:

20 µg/$215

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Human INSR / Insulin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G81377

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

156.33 kDa

Price:

20 µg/$215

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INSR / Insulin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G123453

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

156.33 kDa

Price:

100 µg/$710

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INSR / Insulin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G123330

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

156.33 kDa

Price:

100 µg/$710

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Human INSR / Insulin Receptor Protein (Recombinant DDK (Flag)-His, C-terminus) - LS-G142202

Source:

HEK 293 Cells

Tag:

DDK (Flag)-His, C-terminus

Molecular Weight:

25 kDa

Price:

20 µg/$1,107

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Human INSR / Insulin Receptor Protein (Over-Expression Cell Culture Supernatant 6His, N-terminus) - LS-G26859

Source:

HEK 293 Cells

Tag:

6His, N-terminus

Molecular Weight:

112 kDa

Price:

200 µl/$283

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Human INSR / Insulin Receptor Protein (Recombinant 6His-SUMO, N-terminus) (aa1023-1298) - LS-G56352

Source:

E. coli

Tag:

6His-SUMO, N-terminus

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

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Human INSR / Insulin Receptor Protein (Recombinant His-T7) (aa1024-1298) - LS-G13076

Source:

E. coli

Tag:

His-T7

Molecular Weight:

34.6 kDa

Price:

10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900

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Human INSR / Insulin Receptor Protein (Recombinant His) (aa622-945) - LS-G13082

Source:

E. coli

Tag:

His

Molecular Weight:

38.0 kDa

Price:

10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900

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Rat INSR / Insulin Receptor Protein (Recombinant His) (aa1114-1382) - LS-G13088

Source:

E. coli

Tag:

His

Molecular Weight:

32.3 kDa

Price:

10 µg/$279; 50 µg/$432; 100 µg/$690; 200 µg/$862; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148

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