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HARS

histidyl-tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.

Gene Name: histidyl-tRNA synthetase
Synonyms: HARS, Histidine translase, Histidyl-tRNA synthetase, HRS, USH3B, HisRS
Target Sequences: NM_002109 NP_002100.2 P12081

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Products
Proteins (6)
Over-Expression Lysate (1)
Recombinant (5)
HARS (6)
No (6)
6His, N-terminus (1)
GST (1)
GST, N-terminus (1)
His (1)
His-T7, N-terminus (1)
Myc-DDK (Flag) (1)
Human (5)
Baculovirus (1)
E. coli (2)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (3)
HARS Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
HARS Protein - 12.5% SDS-PAGE of human HARS stained with Coomassie Blue
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Wheat Germ Extract
GST
81.73 kDa
10 µg/$479; 25 µg/$670
HARS Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
57.2 kDa
100 µg/$494
HARS Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Baculovirus
6His, N-terminus
100 µg/$920; 20 µg/$411; 1 mg/$1,808
HARS Protein
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E. coli
His
50 µg/$361; 250 µg/$739; 10 µg/$261
HARS Protein - Recombinant Histidyl tRNA Synthetase (HARS) by SDS-PAGE
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E. coli
His-T7, N-terminus
37.3kDa
10 µg/$268; 50 µg/$362; 200 µg/$722; 100 µg/$577; 1 mg/$1,755; 500 µg/$1,325; 5 mg/$2,708; 2 mg/$1,934
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).