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GLA / Alpha Galactosidase

galactosidase, alpha

GLA / Alpha Galactosidase encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

Gene Name: galactosidase, alpha
Synonyms: GLA, Alpha-D-galactosidase A, Alpha-galactosidase A, Galactosidase, alpha, Melibiase, Alpha-gal A, GALA
Target Sequences: NM_000169 NP_000160.1 P06280

Publications (1)

1
Systemic mRNA Therapy for the Treatment of Fabry Disease: Preclinical Studies in Wild-Type Mice, Fabry Mouse Model, and Wild-Type Non-human Primates. Xuling Zhu, Ling Yin, Matt Theisen, Jenny Zhuo, Summar Siddiqui, Becca Levy, Vladimir Presnyak, Andrea Frassetto, Jaclyn Milton, Timothy Salerno, Kerry E Benenato, Joe Milano, Andy Lynn, Staci Sabnis, Kristine Burke, Gilles Besin, Christine M Lukacs, Lin T Guey, Patrick F Finn, Paolo G V Martini. American journal of human genetics. 2019 Apr;104:625-637. [Full Text Article] [PubMed:30879639] [PMC:PMC6451694] Related Antibodies: LS-B11102.

☰ Filters
Products
Proteins (16)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (3)
Recombinant (12)
GLA / Alpha Galactosidase (16)
SDS-PAGE (1)
No (16)
10His, N-terminus (1)
6His, C-terminus (2)
6His, N-terminus (1)
GST (1)
His, C-terminus (1)
His, N-Terminal (2)
His,C-terminus (1)
His-S (3)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (12)
Macaque (1)
Mouse (2)
293T Cells (1)
E. coli (6)
HEK 293 Cells (6)
Human Cells (1)
Mammalian Cells (1)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (4)
Select
HEK 293 Cells
His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
45.6 kDa
50 µg/$970
GLA / Alpha Galactosidase Protein
Select
HEK 293 Cells
His,C-terminus
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The secreted recombinant human GLA consists of 409 amino acids and has a predicted molecular mass of 46.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 50 kDa band.
50 µg/$395; 500 µg/$1,538
GLA / Alpha Galactosidase Protein - Recombinant Galactosidase Alpha By SDS-PAGE
Select
E. coli
His-S
45.4 kDa
10 µg/$262; 50 µg/$339; 100 µg/$512; 200 µg/$642; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
GLA / Alpha Galactosidase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
10His, N-terminus
48.6 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
GLA / Alpha Galactosidase Protein
Select
E. coli
6His, N-terminus
49.4 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
GLA / Alpha Galactosidase Protein - Recombinant Galactosidase Alpha By SDS-PAGE
Select
E. coli
His-T7
30.3 kDa
50 µg/$432; 10 µg/$279; 200 µg/$862; 100 µg/$690; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148
GLA / Alpha Galactosidase Protein - Recombinant Galactosidase Alpha By SDS-PAGE
Select
E. coli
His-S
31.4 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
GLA / Alpha Galactosidase Protein - Recombinant Galactosidase Alpha By SDS-PAGE
Select
E. coli
His-S
14.8 kDa
10 µg/$262; 50 µg/$339; 100 µg/$512; 200 µg/$642; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
Select
Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
46.39kD
10 µg/$297; 50 µg/$468; 500 µg/$1,585; 1 mg/$2,157
GLA / Alpha Galactosidase Protein - Purified recombinant protein GLA was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
45.3 kDa
20 µg/$1,107
GLA / Alpha Galactosidase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
48.77 kDa
100 µg/$494
Select
293T Cells
Myc-DDK (Flag)
48.77 kDa
20 µg/$150
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
46.4 kDa
10 µg/$384; 50 µg/$557
Select
HEK 293 Cells
His, N-Terminal
~46 kDa
200 µl/$283
Select
HEK 293 Cells
His, N-Terminal
~46 kDa
100 µg/$298
GLA / Alpha Galactosidase Protein - 12.5% SDS-PAGE of human GLA stained with Coomassie Blue
Select
Wheat Germ Extract
GST
72.93 kDa
10 µg/$479; 25 µg/$670
Viewing 1-16 of 16 product results


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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).