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Vector Laboratories, Inc.
6737 Mowry Ave
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FLT3LG / Flt3 Ligand

fms-related tyrosine kinase 3 ligand

Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).

Gene Name: fms-related tyrosine kinase 3 ligand
Synonyms: FLT3LG, FL, Flt3 ligand, FLT3L, SL cytokine
Target Sequences: NM_001459 NP_001450.2 P49771

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Products
Proteins (32)
Over-Expression Lysate (2)
Recombinant (30)
FLT3LG / Flt3 Ligand (32)
SDS-PAGE (2)
Animal-free (1)
No (32)
6His, C-terminus (4)
6His-SUMO, N-terminus (1)
Fc (1)
Fc, C-terminus (2)
GST (1)
GST, N-terminus (1)
His (2)
His, C-Terminal (1)
His, N-Terminal (1)
His,C-terminus (1)
His,N-terminus (1)
Human IgG Fc, C-terminus (1)
Human IgG1 Fc (1)
Myc-DDK (Flag) (3)
Human (20)
Mouse (11)
Rhesus monkey (1)
293T Cells (1)
Baculovirus (2)
CHO Cells (5)
E. coli (11)
HEK 293 Cells (4)
Human Cells (4)
Mammalian Cells (3)
Wheat Germ Extract (2)
Purified (9)
Low endotoxin level (22)
Biologically active (15)
Animal-free (1)
FLT3LG / Flt3 Ligand Protein - Influence of FLT3L treatment on lymphocyte subpopulations. Method: C57BL/6 mice were injected intraperitoneally with 10 ug daily or were left untreated. (A) Percentages of indicated splenic subpopulations in FLT3L KO, WT control or WT-treated mice. (B) Absolute cell numbers of indicated splenic subpopulations in FLT3L KO, WT control, or WT-treated mice.
Select
CHO Cells
Human IgG1 Fc
Less than 0.1 EU/µg protein (determined by LAL method).
10 µg/$351
FLT3LG / Flt3 Ligand Protein
Select
E. coli
None
Biologically active
18.0 kDa
10 µg/$388; 100 µg/$1,359; 500 µg/$2,723
FLT3LG / Flt3 Ligand Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
26.2 kDa
100 µg/$494
FLT3LG / Flt3 Ligand Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
26.2 kDa
20 µg/$150
Select
Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
18.9kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
18.6 kDa
2 µg/$268; 10 µg/$349
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
17.6 kDa
2 µg/$268; 10 µg/$349
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
2 µg/$281; 10 µg/$369
FLT3LG / Flt3 Ligand Protein
Select
E. coli
None
Biologically active
17.6 kDa
10 µg/$388; 100 µg/$1,186; 500 µg/$2,269
FLT3LG / Flt3 Ligand Protein - Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3. The ED50 for this effect is typically 2-11 ng/mL.
Select
Baculovirus
His,N-terminus
SDS-PAGE
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant human FLT3LG consists of 175 amino acids and predicts a molecular mass of 20.2 kDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
10 µg/$286; 1 mg/$2,344; 50 µg/$364
FLT3LG / Flt3 Ligand Protein - Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3. The ED50 for this effect is typically 7-30 ng/mL.
Select
HEK 293 Cells
Human IgG Fc, C-terminus
SDS-PAGE
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant mouse FLT3L /Fc is a disulfide-linked homodimer. The reduced monomer comprises 403 amino acids and has a predicted molecular mass of 45.4 kDa. The apparent molecular mass of the protein is approximately 56 and 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
20 µg/$317; 100 µg/$480; 1 mg/$2,344
FLT3LG / Flt3 Ligand Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Select
Human Cells
Fc
Less than 1.0 EU/µg protein (determined by LAL method).
45.4 kDa
20 µg/$443
FLT3LG / Flt3 Ligand Protein - Purified recombinant protein FLT3LG was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
26.2 kDa
20 µg/$1,107
FLT3LG / Flt3 Ligand Protein - Recombinant FMS Like Tyrosine Kinase 3 Ligand By SDS-PAGE
Select
E. coli
His
22.2 kDa
10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204
Select
CHO Cells
None
Biologically active
Less than 0.2 EU/µg protein (determined by LAL method).
18~22 kDa (SDS-PAGE)
50 µg/$432; 10 µg/$296; 1 mg/$1,853
FLT3LG / Flt3 Ligand Protein - 12.5% SDS-PAGE of human FLT3LG stained with Coomassie Blue
Select
Wheat Germ Extract
GST
52.8 kDa
10 µg/$479; 25 µg/$670
FLT3LG / Flt3 Ligand Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
Select
Mammalian Cells
Fc, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
45.5 kDa
10 µg/$288; 50 µg/$436
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
18.9 kDa
10 µg/$288; 50 µg/$436
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
19.4 kDa
10 µg/$297; 50 µg/$460
FLT3LG / Flt3 Ligand Protein - Induction of IL-6 in mouse myeloid leukemia M1 cells by human FLT3L.
Select
CHO Cells
None
Biologically active
Less than 0.01 ng/µg (determined by LAL method).
The 159 amino acid recombinant protein has a predicted molecular mass of approximately 18 kD. The DTT-reduced and non-reduced protein migrate at approximately 23-33 kD and 20-30 kD by SDS-PAGE. The N-terminal amino acid is Thr.
10 µg/$352; 25 µg/$436; 100 µg/$951; 500 µg/$2,031
FLT3LG / Flt3 Ligand Protein - Induction of IL-6 in mouse myeloid leukemia M1 cells by mouse FLT3L.
Select
CHO Cells
None
Biologically active
Less than 0.01 ng/µg (determined by LAL method).
The 162 amino acid recombinant protein has a predicted molecular mass of approximately 18.3 kD. The DTT-reduced and non-reduced protein migrate at approximately 20-26 kD and 24-30 kD by SDS-PAGE. The N-terminal amino acid is Gly.
10 µg/$352; 25 µg/$436; 100 µg/$951
Select
Baculovirus
His
Less than 1.0 EU/µg protein (determined by LAL method).
20.2 kDa
10 µg/$382; 50 µg/$525
FLT3LG / Flt3 Ligand Protein
Select
E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
17.6 kDa
100 µg/$562
Viewing 1-25 of 32 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).