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FBL / FIB / Fibrillarin

fibrillarin

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).

Gene Name: fibrillarin
Synonyms: FBL, FLRN, FBRL, FIB1, Fibrillarin, RNU3IP1, FIB
Target Sequences: NM_001436 NP_001427.2 P22087

Publications (3)

1
Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse. Beyret E, Lin H. Developmental biology. 2011 355:215-26. (IHC-Fr; Mouse) [PubMed:21539824] [PMC:PMC3443393]
2
The chemotherapeutic agent CX-5461 irreversibly blocks RNA polymerase I initiation and promoter release to cause nucleolar disruption, DNA damage and cell inviability. Jean-Clément Mars, Michel G Tremblay, Mélissa Valere, Dany S Sibai, Marianne Sabourin-Felix, Frédéric Lessard, Tom Moss. NAR cancer. 2020 December;2:zcaa032. [Full Text Article] [PubMed:33196044] [PMC:PMC7646227]
3
Ribosomal DNA promoter recognition is determined in vivo by cooperation between UBTF1 and SL1 and is compromised in the UBTF-E210K neuroregression syndrome. Michel G Tremblay, Dany S Sibai, Melissa Valère, Jean-Clément Mars, Frédéric Lessard, Roderick T Hori, Mohammad Moshahid Khan, Victor Y Stefanovsky, Mark S LeDoux, Tom Moss. PLoS genetics. 2022 February;18:e1009644. [Full Text Article] [PubMed:35139074] [PMC:PMC8863233]

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Proteins (8)
Over-Expression Lysate (2)
Recombinant (6)
FBL / FIB / Fibrillarin (8)
No (8)
6His-SUMO, N-terminus (1)
GST (1)
His (1)
His-T7, N-terminus (2)
Myc-DDK (Flag) (3)
Arabidopsis thaliana (1)
Human (5)
Rat (1)
293T Cells (1)
E. coli (4)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (3)
FBL / FIB / Fibrillarin Protein
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E. coli
His
100 µg/$411; 500 µg/$985; 20 µg/$276
FBL / FIB / Fibrillarin Protein - Recombinant Fibrillarin (FBL) by SDS-PAGE
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E. coli
His-T7, N-terminus
28.7kDa
10 µg/$267; 50 µg/$352; 200 µg/$705; 100 µg/$565; 1 mg/$1,728; 500 µg/$1,303; 5 mg/$2,680; 2 mg/$1,906
FBL / FIB / Fibrillarin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
33.6 kDa
100 µg/$494
FBL / FIB / Fibrillarin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
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293T Cells
Myc-DDK (Flag)
33.6 kDa
20 µg/$150
FBL / FIB / Fibrillarin Protein - Recombinant Fibrillarin (FBL) by SDS-PAGE
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E. coli
His-T7, N-terminus
24.5kDa
10 µg/$272; 50 µg/$382; 200 µg/$765; 100 µg/$612; 1 mg/$1,822; 500 µg/$1,383; 5 mg/$2,781; 2 mg/$2,004
FBL / FIB / Fibrillarin Protein - 12.5% SDS-PAGE of human FBL stained with Coomassie Blue
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Wheat Germ Extract
GST
61.05 kDa
10 µg/$479; 25 µg/$670
FBL / FIB / Fibrillarin Protein - Purified recombinant protein FBL was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
33.6 kDa
20 µg/$1,107
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).