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EPO / Erythropoietin

erythropoietin

is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Gene Name: erythropoietin
Family/Subfamily: Hormone , not assigned-Hormone
Synonyms: EPO, EP, MVCD2, Erythropoietin
Target Sequences: NM_000799 NP_000790.2 P01588

Publications (5)

1
Immunomagnetic beads-based isolation of erythropoietins from urine and blood for sports anti-doping control. Desharnais P, Naud JF, Ayotte C. Drug testing and analysis. 2017 9:1744-1752. (WB; Human) [Full Text Article] [PubMed:28994210]
2
High-Iron Consumption Impairs Growth and Causes Copper-Deficiency Anemia in Weanling Sprague-Dawley Rats. Ha JH, Doguer C, Wang X, Flores SR, Collins JF. PloS one. 2016 August;11:e0161033. [Full Text Article] [PubMed:27537180] [PMC:PMC4990348]
3
Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system. Philippe Desharnais, Jean-François Naud, Christiane Ayotte. Drug testing and analysis. 2018 Nov;;10:1698-1707. [Full Text Article] [PubMed:30365250] Related Antibodies: LS-C305828, LS-C23228, LS-C521874.
4
Wuzi-Yanzong prescription alleviates spermatogenesis disorder induced by heat stress dependent on Akt, NF-?B signaling pathway. Su-Qin Hu #, Dian-Long Liu #, Chun-Rui Li, Ya-Hui Xu, Ke Hu, Li-Dan Cui, Jian Guo. Scientific reports. 2021 Sep;11:18824. [Full Text Article] [PubMed:34552120] [PMC:PMC8458393] Related Antibodies: LS-B15015.
5
Comparison and optimization of SAR-PAGE tests for erythropoietins in doping analysis. Xinmiao Zhou, Lisi Zhang, Sen He, Li Shen, Chunji He. Drug testing and analysis. 2020 Janurary;12:109-118. [Full Text Article] [PubMed:31668004]
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Proteins (38)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (3)
Recombinant (34)
EPO / Erythropoietin (38)
Bind (1)
ELISA (1)
Func (1)
SDS-PAGE (3)
No (38)
6His, C-terminus (1)
6His, N-terminus (2)
6His-B2M, N-terminus (1)
6His-SUMO, N-terminus (1)
GST (1)
His (4)
His, C-Terminal (1)
His, N-Terminal (4)
His,C-terminus (1)
His-GST (2)
His-S (2)
His-SUMO (2)
His-T7 (2)
Human IgG Fc, C-terminus (1)
Human Mutant IgG1 Fc (1)
Myc-DDK (Flag) (2)
Bovine (2)
Crab-eating macaque (1)
Cynomolgus monkey (1)
Horse (1)
Human (14)
Mouse (7)
Pig (8)
Rat (3)
293F Cells (1)
293T Cells (1)
CHO Cells (6)
E. coli (11)
HEK 293 Cells (9)
Human (3)
Mammalian Cells (1)
Wheat Germ Extract (1)
Yeast (4)
Purified (11)
Supernatant (1)
Low endotoxin level (12)
Biologically active (10)
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Select
E. coli
His-GST
50.8 kDa
50 µg/$382; 10 µg/$271; 200 µg/$762; 100 µg/$610; 1 mg/$1,817; 500 µg/$1,378; 5 mg/$2,775; 2 mg/$1,998
Select
CHO Cells
Human Mutant IgG1 Fc
Less than 0.06 EU/µg protein (determined by LAL method).
50 µg/$463
EPO / Erythropoietin Protein
Select
Yeast
6His, N-terminus
20.23 kD
100 µg/$570; 20 µg/$395; 1 mg/$1,973
EPO / Erythropoietin Protein
Select
E. coli
His-SUMO
34.32 kD
1 mg/$1,679; 100 µg/$598; 20 µg/$405
EPO / Erythropoietin Protein - 12.5% SDS-PAGE of human EPO stained with Coomassie Blue
Select
Wheat Germ Extract
GST
47.7 kDa
10 µg/$479; 25 µg/$670
EPO / Erythropoietin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Select
E. coli
His-S
18.0 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
Select
E. coli
His-S
18.0 kDa
10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Select
E. coli
His-T7
16.1 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Select
E. coli
His-GST
51.0 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Select
E. coli
His-T7
22.3 kDa
10 µg/$284; 50 µg/$460; 100 µg/$735; 200 µg/$917; 1 mg/$2,037; 500 µg/$1,571; 5 mg/$3,207; 2 mg/$2,225
Select
None
Biologically active
50 µg/$484
EPO / Erythropoietin Protein - Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL.
Select
HEK 293 Cells
None
SDS-PAGE
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant mouse Epo consists 166 amino acids and predicts a molecular mass of 18.6 kDa.
20 µg/$317
EPO / Erythropoietin Protein - Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.
Select
HEK 293 Cells
His,C-terminus
Bind ELISA Func SDS-PAGE
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
50 µg/$403
EPO / Erythropoietin Protein
Select
HEK 293 Cells
Human IgG Fc, C-terminus
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant human EPO consists 404 amino acids and predicts a molecular mass of 45.1 kDa.
1 mg/$2,344; 100 µg/$480
EPO / Erythropoietin Protein - Eukaryotic Erythropoietin By SDS-PAGE
Select
Human
None
20kDa
50 µg/$762; 10 µg/$332; 200 µg/$1,378; 100 µg/$1,167; 1 mg/$2,664; 500 µg/$2,160; 5 mg/$5,321; 2 mg/$2,839
Select
Human
His, C-Terminal
Less than 1.0 EU/µg protein (determined by LAL method).
19.2kD
10 µg/$262; 50 µg/$365; 1 mg/$2,157; 500 µg/$1,585
EPO / Erythropoietin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
21.31 kDa
100 µg/$494
EPO / Erythropoietin Protein
Select
HEK 293 Cells
His, N-Terminal
~19 kDa (30-40 kDa reducing conditions)
10 µg/$289; 100 µg/$432; 250 µg/$726; 50 µg/$360
Select
HEK 293 Cells
His, N-Terminal
~19 kDa
200 µl/$283
Select
HEK 293 Cells
His, N-Terminal
~19 kDa
100 µg/$298
EPO / Erythropoietin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
21.31 kDa
20 µg/$150
Select
E. coli
165 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
18.26 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
EPO / Erythropoietin Protein - Recombinant Mouse Erythropoietin/EPO (C-6His)
Select
Human
6His, C-terminus
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
19.4 kDa
10 µg/$388; 50 µg/$875
EPO / Erythropoietin Protein
Select
HEK 293 Cells
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
~20 kDa
50 µg/$282; 1 mg/$1,021
Viewing 1-25 of 38 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).