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EIF4E

eukaryotic translation initiation factor 4E

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Gene Name: eukaryotic translation initiation factor 4E
Synonyms: EIF4E, EIF-4E, EIF4E1, EIF4EL1, EIF4F, CBP, EIF-4F 25 kDa subunit, MRNA cap-binding protein
Target Sequences: NM_001968 NP_001959.1 P06730

Publications (2)

1
Circadian proteins CLOCK and BMAL1 in the chromatoid body, a RNA processing granule of male germ cells. Peruquetti RL, de Mateo S, Sassone-Corsi P. PloS one. 2012 7:e42695. [PubMed:22900038] [PMC:PMC3416844]
2
Licochalcone A inhibits EGFR signalling and translationally suppresses survivin expression in human cancer cells. Feng Gao, Ming Li, Xinfang Yu, Wenbin Liu, Li Zhou, Wei Li. Journal of cellular and molecular medicine. 2020 November; [Full Text Article] [PubMed:33247550] [PMC:PMC7812290] Related Antibodies: LS-B12932.

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Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
EIF4E (7)
No (7)
GST (1)
His (1)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (2)
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E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
25.1kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,353
EIF4E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
24.9 kDa
20 µg/$150
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
25.1 kDa
10 µg/$317; 50 µg/$524
EIF4E Protein
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E. coli
His
50 µg/$411; 250 µg/$985; 10 µg/$276
EIF4E Protein - Purified recombinant protein EIF4E was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
24.9 kDa
20 µg/$1,107
EIF4E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
24.9 kDa
100 µg/$494
EIF4E Protein - 12.5% SDS-PAGE of human EIF4E stained with Coomassie Blue
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Wheat Germ Extract
GST
49.61 kDa
10 µg/$479; 25 µg/$670
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).