EIF2AK2 / PKR

eukaryotic translation initiation factor 2-alpha kinase 2

IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.

☰ Filters

Products

Proteins (14)

Type

Over-Expression Lysate (6)

Recombinant (8)

Target

EIF2AK2 / PKR (14)

Publications

No (14)

Tag

6His, N-terminus (2)

GST, N-terminus (1)

His, N-Terminal (2)

His-SUMO (1)

His-T7 (1)

Myc-DDK (Flag) (7)

Species

Human (9)

Mouse (1)

Rat (1)

Source

293T Cells (3)

E. coli (5)

HEK 293 Cells (4)

Wheat Germ Extract (1)

Yeast (1)

Purification

Purified (3)

Select

Human EIF2AK2 / PKR Protein (Recombinant 6His, N-terminus) (Full Length) - LS-G22902

Source:

Yeast

Tag:

6His, N-terminus

Molecular Weight:

63.96 kD

Price:

100 µg/$446; 20 µg/$336; 1 mg/$1,460

Select

Human EIF2AK2 / PKR Protein (Recombinant His-SUMO) (Full Length) - LS-G20694

Source:

E. coli

Tag:

His-SUMO

Molecular Weight:

77.92 kD

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

Select

Mouse EIF2AK2 / PKR Protein (Recombinant His, N-Terminal) (aa205-495) - LS-G12276

Source:

E. coli

Tag:

His, N-Terminal

Molecular Weight:

37.2 kDa

Price:

50 µg/$432; 10 µg/$279; 200 µg/$862; 100 µg/$690; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148

Select

Human EIF2AK2 / PKR Protein (Recombinant 6His, N-terminus) (aa224-502) - LS-G24042

Source:

E. coli

Tag:

6His, N-terminus

Molecular Weight:

35.6 kDa

Price:

10 µg/$279; 50 µg/$432; 100 µg/$690; 200 µg/$862; 1 mg/$1,962; 500 µg/$1,505; 5 mg/$3,011; 2 mg/$2,148

Select

Human EIF2AK2 / PKR Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G66086

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

61.9 kDa

Price:

20 µg/$1,107

Select

Human EIF2AK2 / PKR Protein (Recombinant GST, N-terminus) (aa1-100) - LS-G59758

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

Select

EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G99861

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

61.9 kDa

Price:

100 µg/$494

Select

Human EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G76877

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

61.9 kDa

Price:

20 µg/$150

Select

Human EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G76879

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

57.2 kDa

Price:

20 µg/$150

Select

Human EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G76878

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

61.9 kDa

Price:

20 µg/$150

Select

EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G125493

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

61.9 kDa

Price:

100 µg/$494

Select

EIF2AK2 / PKR Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G125494

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

57.2 kDa

Price:

100 µg/$494

Select

Human EIF2AK2 / PKR Protein (Recombinant His, N-Terminal) - LS-G132837

Source:

E. coli

Tag:

His, N-Terminal

Molecular Weight:

32kDa

Price:

10 µg/$377; 50 µg/$942; 200 µg/$1,600; 100 µg/$1,367; 1 mg/$3,009; 500 µg/$2,423; 5 mg/$6,581; 2 mg/$3,511

Select

Rat EIF2AK2 / PKR Protein (Recombinant His-T7) (aa279-506) - LS-G12277

Source:

E. coli

Tag:

His-T7

Molecular Weight:

29.9 kDa

Price:

10 µg/$282; 50 µg/$447; 100 µg/$715; 200 µg/$892; 1 mg/$2,005; 500 µg/$1,543; 5 mg/$3,123; 2 mg/$2,192

Viewing 1-14 of 14 product results

(0) Selected Add Selected To Cart Compare Selected Email Selected Clear Selected Top Of Search

---

Filtered By:

Products: Proteins

Clear Filters

If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.