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DLL4

delta-like 4 (Drosophila)

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate.

Gene Name: delta-like 4 (Drosophila)
Synonyms: DLL4, Delta-like 4 protein, Delta like 4, Delta-like 4 (Drosophila), Delta-like 4 homolog, Drosophila Delta homolog 4, Notch ligand DLL4, Delta 4, Delta ligand 4, Delta-like protein 4, Delta4, Hdelta2, Notch ligand delta-2
Target Sequences: NM_019074 NP_061947.1 Q9NR61

Publications (2)

1
Anthrax toxins cooperatively inhibit endocytic recycling by the Rab11/Sec15 exocyst. Guichard A, McGillivray SM, Cruz-Moreno B, van Sorge NM, Nizet V, Bier E. Nature. 2010 467:854-8. (ICC; Human) [PubMed:20944747] Related Antibodies: LS-C19035.
2
Cholera toxin disrupts barrier function by inhibiting exocyst-mediated trafficking of host proteins to intestinal cell junctions. Guichard A, Cruz-Moreno B, Cruz-Moreno BC, Aguilar B, van Sorge NM, Kuang J, Kurkciyan AA, Wang Z, Hang S, Pineton de Chambrun GP, McCole DF, Watnick P, Nizet V, Bier E. Cell host & microbe. 2013 Sep;14:294-305. [Full Text Article] [PubMed:24034615] [PMC:PMC3786442] Related Antibodies: LS-C19035.

☰ Filters
Products
Proteins (9)
Recombinant (9)
DLL4 (9)
No (9)
Fc (1)
Fc, C-terminus (1)
His (1)
His, C-Terminal (1)
His, N-Terminal (1)
His-GST (1)
Human IgG1 Fc (2)
Human (6)
Mouse (1)
Rat (2)
E. coli (3)
HEK 293 Cells (4)
Human (1)
Human Cells (1)
Purified (2)
Low endotoxin level (6)
DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
Select
HEK 293 Cells
Human IgG1 Fc
Less than 0.01 EU/µg protein (determined by LAL method).
~80kDa (SDS-PAGE)
50 µg/$519; 10 µg/$379
Select
Human Cells
Fc
Less than 1.0 EU/µg protein (determined by LAL method).
81 kDa
50 µg/$483
DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Select
HEK 293 Cells
Human IgG1 Fc
Less than 0.1 EU/µg protein (determined by LAL method).
~95kDa (SDS-PAGE)
50 µg/$379; 10 µg/$288
Select
HEK 293 Cells
None
Less than 0.1 ng/µg of protein (less than 1EU/µg).
54.0 kDa
5 µg/$268; 25 µg/$349
DLL4 Protein - Recombinant Delta Like Protein 4 (dLL4) by SDS-PAGE
Select
E. coli
His, N-Terminal
23.4kDa
10 µg/$267; 50 µg/$352; 200 µg/$702; 100 µg/$562; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
Select
Human
His, C-Terminal
Less than 1.0 EU/µg protein (determined by LAL method).
55.7kD
50 µg/$395; 500 µg/$1,585; 1 mg/$2,157; 10 µg/$277
DLL4 Protein - Recombinant Delta Like Protein 4 By SDS-PAGE
Select
E. coli
His-GST
39.9 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
DLL4 Protein - Recombinant Delta Like Protein 4 By SDS-PAGE
Select
E. coli
His
14.3 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
DLL4 Protein - Recombinant Human Delta-like Protein 4/DLL4 (C-Fc)
Select
HEK 293 Cells
Fc, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
85-100 kDa
10 µg/$266
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).